文献类型：期刊文献

英文题名：Mass cultivation of marine fish Chinook salmon embryo cells in bioreactor with low-serum medium

作者：Chen, JX; Sun, XM; Zhang, L; Yi, XP; Zhang, YX

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：249

期号：1-4

起止页码：35

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000231765000004)】；

语种：英文

外文关键词：animal cell culture; bioreactor; CHSE cell; marine fish cell

摘要：Mass cultivation of marine fish cells is the key step of killed virus vaccine preparation for preventing mariculture fishes from virus infection. In the low-serum culture of Chinook salmon embryo cells (CHSE), the optimal glucose (1-5 mmol/L) and glutamine (0-0.6 mmol/L) concentrations were determined and the culture technique of medium-exchange was established in the basis on the understanding of cell growth and metabolism features. CHSE cells attached on microcarriers were inoculated into 1.5-L bioreactor at 6.5 x 10(5) cells/mL and the cells of 2.3 x 10(6) cells/mL in mid logarithmic phase at 9 days were successfully transferred to a 5-L disk packed-bed bioreactor at the density of 5.3 x 10(5) cells/mL. A cell density of 5.9 x 10(6) cells/mL was achieved at 22 days in the packed-bed bioreactor using medium-exchange culture to control nutrient and byproduct concentrations away from inhibitory levels. In addition, cell density in packed-bed bioreactor was successfully estimated by the glucose consumption kinetics. This work laid the foundations for large-scale preparation of viruses by CHSE cells as host. (c) 2005 Elsevier B.V. All rights reserved.