文献类型：期刊文献

英文题名：hypoxia-activated near infrared fluorescent probe for cyclooxygenase-2 and in vivo imaging for tumor and inflammation

作者：Ti, Yuezheng[1];Yu, Ling[2];Tang, Yao[1];Jin, Tongxia[1];Yang, Ming[1];Wang, Rui[2];Xu, Yufang[1];Zhu, Weiping[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：265

起止页码：582

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000430232500070)】；

基金：This work was supported by the National Natural Science Foundation of China (Grants 21476077, 21236002) and Shanghai Pujiang Program. W. Zhu is grateful for the support from Chinese Scholarship Council. We also very much appreciate the suggestions from the reviewers.

语种：英文

外文关键词：Fluorescent probe; Cyclooxygenase-2; Tumor; Hypoxia; Inflammation

摘要：Tumor is one of the most serious diseases, and its early diagnosis is of great significance for improving the prognosis and patient survival rate. Herein, aiming at the detection of cyclooxygenase-2 (COX-2), which plays an important role in tumorigenesis, we synthesized a NIR fluorescent probe YZP1, by using near infrared fluorescent dye Cy5.5 as the fluorophore and indomethacin (IMC) as the targeting group. Due to the quenching effect of azo group, the fluorescence of YZP1 was quenched. Under hypoxia condition and the reduction of Cytochrome P450 reductase, the azo bond of YZP1 was broken, and the fluorescence quantum yield increased from 0.0028 to 0.125. Furthermore, when HeLa cells were co-incubated with YZP1 under hypoxia condition, remarkable red fluorescence were observed, and the ratio of fluorescence intensity between hypoxic and normoxic cells could reach 9 folds. After treated with the celecoxib, an inhibitor of COX-2, of different concentrations, the fluorescence will be weakened gradually, which verify the binding of YZP1 and COX-2. Finally, by using mouse inflammation model and tumor xenograft model, the in vivo imaging ability of YZP1 was also demonstrated. (C) 2018 Elsevier B.V. All rights reserved.