文献类型：期刊文献

英文题名：EsrB negatively regulates expression of the glutamine sythetase GlnA in the fish pathogen Edwardsiella piscicida

作者：Guan, Yunpeng[1];Yin, Kaiyu[1];Zhou, Mian[1,2,3];Yang, Minjun[4];Zhang, Yuanxing[1,2,3];Liu, Xiaohong[1,2,3];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 200237, Peoples R China

年份：2018

卷号：365

期号：4

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000429769700008)】；

基金：The study was supported by National Natural Science Foundation of China, Nos. 31430090 (YXZ), 31602200 (XHL) and 31400122 (MJY), the Ministry of Agriculture of China, No. CARS-47 (QYW), and Shanghai Pujiang Program, No. 16PJD018 (QYW).

语种：英文

外文关键词：comparative proteomics; EsrB; Edwardsiella piscicida; glnA

摘要：Edwardsiella piscicida is a gram-negative bacterial pathogen invading a wide range of fish species. Response regulator EsrB is essential for the activation of type III and type VI secretion systems (T3/T6SS). In this study, proteomes of the wild-type E. piscicida EIB202 and Delta esrB mutant strains were compared to identify the regulon components of EsrB cultured in DMEM allowing T3/T6SS expression. As a result, 19 proteins showed different expression, which were identified to be associated with T3/T6SS, related to amino acid transport and metabolism, and energy production. Particularly, GlnA, a glutamine synthetase essential for ammonia assimilation and glutamine biosynthesis from glutamate, was found to be regulated negatively by EsrB. Moreover, GlnA affected bacterial growth in vitro and bacterial colonization in vivo. Collectively, our results indicated that EsrB plays important roles in regulating the expression of metabolic pathways and virulence genes, including glutamine biosynthesis in E. piscicida during infection.