文献类型：期刊文献

英文题名：Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin

作者：Lv, Xin[1]; Dai, Lu[1]; Bai, Fangmin[1]; Wang, Zhanqing[1]; Zhang, Liaoyuan[2]; Shen, Yaling[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai, 200237, China; [2] Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China

年份：2016

卷号：3

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513086574)

语种：英文

外文关键词：Glycerol - Ketones - Liquid crystals

摘要：Background: Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites. Results: In this study, slaC and gldA encoding meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) and glycerol dehydrogenase (GDH), respectively, in S. marcescens MG1 were knocked out to block the conversion from AC to 2,3-butanediol (2,3-BD). The resulting strain MG14 was found to produce a large amount of optically pure (3R)-AC with a little 2,3-BD, indicating that another enzyme responsible for 2,3-BD formation except meso-2,3-BDH and GDH existed in the strain MG1. Furthermore, SlaR protein, a transcriptional activator of AC cluster, was overexpressed using PC promoter in the strain MG14, leading to enhancement of the (3R)-AC yield by 29.91%. The recombinant strain with overexpression of SlaR, designated as S. marcescens MG15, was used to perform medium optimization for improving (3R)-AC production. Conclusion: Under the optimized conditions, 39.91±1.35g/l (3R)-AC was produced by strain MG15 with the productivity of 1.11g/lh and the conversion rate of 80.13%. ? 2016, The Author(s).