文献类型：期刊文献

英文题名：Strategy for the improvement of prodigiosin production by a Serratia marcescens mutant through fed-batch fermentation

作者：Tao, JL; Wang, XD; Shen, YL; Wei, DZ

机构：[1]E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2005

卷号：21

期号：6-7

起止页码：969

外文期刊名：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000233385500027)】；

语种：英文

外文关键词：feeding strategy; mutation; prodigiosin; Serratia marcescens; ultra violet

摘要：A Serratia marcescens mutant for prodigiosin production was obtained by u.v. mutation with rational screening methods and a two-step feeding strategy was used to increase its productivity. In flasks, the mutant strain B6 gave a 2.8-fold higher prodigiosin production than that of the parent strain with glycerol as a carbon source. In a 5-l bioreactor, with a two-step feeding strategy in which glucose was selected as the initial carbon source in the fermentation media and glycerol was fed as a 'prodigiosin inducer', it gave a 7.8 times higher prodigiosin production (583 mg/l) than the parent stain with the original cultivation mode.