文献类型：期刊文献

中文题名：培养方式对真皮组织体外构建的影响

英文题名：Effects of the Culture Method on the Construction of Dermal Substitutes in vitro

作者：何燕娜[1];丁春梅[1];江华[1];周燕[1];谭文松[1]

机构：[1]华东理工大学生物反应工程国家重点实验室,上海200237

年份：2007

卷号：27

期号：5

起止页码：75

中文期刊名：中国生物工程杂志

外文期刊名：China Biotechnology

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目(20576036)

语种：中文

中文关键词：组织工程;真皮;转瓶培养

外文关键词：Tissue engineering Dermal Spinner flask

摘要：采用静态培养和转瓶培养方式分别构建真皮组织,考察培养方式和搅拌转速对细胞在三维支架材料中增殖、代谢、分布的影响。结果表明,由转瓶培养方式构建的细胞-材料复合物,其最终细胞密度和细胞比生长速率均明显高于静态培养(14.2～27.6×106cells/cm3vs10.1×106cells/cm3和0.145～0.262d-1vs0.111d-1),而转速达80r/min的转瓶尤其突出;静态培养的细胞-材料复合物内部细胞稀少,且分布不均匀,转瓶培养的细胞-材料复合物在材料表面和内部细胞密度都有所提高,分布情况也得到改善,且80r/min转瓶培养的组织其细胞密度和分布均优于10r/min和40r/min转瓶培养。转瓶培养在其转速达到一定强度时能明显提高细胞在支架中的增殖速率,缩短培养时间,并有效改善细胞在支架内的分布,是一种理想的培养方式。
Culture environment is the key factor in the construction of dermal skin. It was investigated that the effects of the culture methods, including the static culture and spinner flask culture, and stir speeds on the cells proliferation, metabolism and distribution within collagen-chitosan sponges. A higher cell density and specific growth rate was obtained with spinner flask culture versus static culture, especially, the 80 r/min spinner flask culture. The cell distribution in dermal substitutes from stirred culture system was more uniform than static culture, as well as that with increase of stir speeds in spinner flask. In summary, the spinner flasks culture with proper stir speed shows promise for the construction of dermal substitutes in vitro.