文献类型：期刊文献

中文题名：电化学限域SiN_(X)纳米孔道检测核酸分子的研究

英文题名：Detection of single oligonucleotide with an electrochemical confined solid-state nanopore

作者：李巧[1];林瑶[1];应佚伦[1];刘少创[1];龙亿涛[1]

机构：[1]结构可控先进功能材料及其制备教育部重点实验室,华东理工大学化学与分子工程学院,上海200237

年份：2017

卷号：47

期号：12

起止页码：1445

中文期刊名：中国科学:化学

外文期刊名：SCIENTIA SINICA Chimica

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；PubMed;

基金：国家自然科学基金(编号:21505043,21421004,21327807);中央高校基本科研业务费(编号:222201718001,222201717003,222201714012)资助项目

语种：中文

中文关键词：固体纳米孔道;电化学空间限域;核酸分子;单分子分析

外文关键词：solid-state nanopore;electrochemical confined space;oligonucleotides;single-molecule analysis

摘要：固体纳米孔道因其机械强度高、尺寸可控、易于表面修饰及集成化设计等优点被广泛应用于DNA、RNA和蛋白质等生物分子的检测研究.为了检测单个单链核酸分子,本研究采用电化学刻蚀法可控制备了单个SiN_(X)固体纳米孔道,通过SiN_(X)固体纳米孔道限域空间效应增强了纳米孔道与短链核酸分子之间的弱相互作用,从而实现了核酸分子的单分子水平检测.通过研究不同孔径(3.1和8.5 nm)纳米孔道与核酸分子间的弱相互作用差异,有效区分了核酸分子在限域空间内产生的过孔和碰撞两种个体行为,加深了对固体纳米孔道限域空间内核酸分子电化学行为的理解.
Solid-state nanopore has been widely used for a variety of single-molecule analysis due to its advantage of size control,high stability,adjustable surface properties,and the potential of further integration.Therefore,the insensitive studies have applied for the nanopore analysis of DNA,RNA and proteins.In order to detect an oligonucleotide at single-molecule level,here,we fabricated two SiN_(X)solid-state nanopores with diameter of 3.1 and 8.5 nm by controlled dielectric breakdown.The weak interaction between the solid-state nanopore and the oligonucleotide could be efficiently enhanced by electrochemical confined space effect of nanopore.We employed a 67-nt oligonucleotide as a model which could be electrochemically driven into a nanopore.The model ssDNA in nanopore with confined diameter of 3.1 nm could result in two kinds of electrochemical behaviors which assign to bumping and translocation.Our findings pave the path for a better understanding of the electrochemical behavior of the single molecule in a confined space.