文献类型：期刊文献

英文题名：Paper-Based Microfluidic Device for Extracellular Lactate Detection

作者：Nan, Yan[1];Zuo, Peng[1];Ye, Bangce[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2024

卷号：14

期号：9

外文期刊名：BIOSENSORS-BASEL

收录：;EI(收录号：20244017137077);WOS:【SCI-EXPANDED(收录号:WOS:001324007900001)】；

基金：This research was funded by National Key Research and Development Program of China grant number 2023YFC3205801, 2023YFC2812101 and Shanghai Beyond Limits Manufacturing Project.

语种：英文

外文关键词：paper-based microfluidic; lactate detection; colorimetric analysis; tumor cell; drug screening

摘要：Lactate is a critical regulatory factor secreted by tumors, influencing tumor development, metastasis, and clinical prognosis. Precise analysis of tumor-cell-secreted lactate is pivotal for early cancer diagnosis. This study describes a paper-based microfluidic chip to enable the detection of lactate levels secreted externally by living cells. Under optimized conditions, the lactate biosensor can complete the assay in less than 30 min. In addition, the platform can be used to distinguish lactate secretion levels in different cell lines and can be applied to the screening of antitumor drugs. Through enzymatic chemical conversion, this platform generates fluorescent signals, enabling qualitative assessment under a handheld UV lamp and quantitative analysis via grayscale intensity measurements using ImageJ (Ver. 1.50i) software. The paper-based platform presented in this study is rapid and highly sensitive and does not necessitate other costly and intricate instruments, thus making it applicable in resource-constrained areas and serving as a valuable tool for investigating cell lactate secretion and screening various anti-cancer drugs.