文献类型：期刊文献

中文题名：Rational design of shortwave infrared(SWIR) fluorescence probe:Cooperation of ICT and ESIPT processes for sensing endogenous cysteine

作者：Maoju Chang[1];Chenxu Yan[1];Lei Shi[1];Dan Li[1];Wei Fu[1];Zhiqian Guo[1]

机构：[1]Shanghai Key Laboratory of Functional Materials Chemistry,Key Laboratory for Advanced Materials and Institute of Fine Chemicals,Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Feringa Nobel Prize Scientist Joint Research Center,Frontiers Science Center for Materiobiology and Dynamic Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China

年份：2022

卷号：33

期号：2

起止页码：762

中文期刊名：Chinese Chemical Letters

外文期刊名：中国化学快报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：supported by the National Natural Science Foundation of China (Nos.21878087,21908060);the Innovation Program of Shanghai Municipal Education Commission,Shuguang Program (No.18SG27);the NIH guidelines for the care and use of laboratory animals (NIH Publication No.85-23,Rev.1985);approved by the Institutional Animal Care and Use Committee of National Tissue Engineering Center (Shanghai,China)。

语种：英文

中文关键词：Fluorescence probe;Shortwave infrared;Intramolecular charge transfer;Excited-state intermolecular proton transfer;Near-infrared

摘要：Cysteine is well-known to be an important biothiol and related to many diseases. However, the in vivo detection of endogenous cysteine still suffers from lacking small-molecule fluorophores with both excitation and emission in the near-infrared(650-900 nm)/shortwave-infrared region. Herein, we report a molecular engineering strategy for shortwave infrared(SWIR, 900-1700 nm) sensing of cysteine, which integrated an excited-state intermolecular proton transfer(ESIPT) building block into the intramolecular charge transfer(ICT) scaffold. The obtained novel fluorophore SH-OH displays a maximum absorption at the NIR region, and emission at the SWIR region. We introduce the cysteine-recognition moiety to SH-OH structure, and demonstrate sensing of endogenous cysteine in living animals, using the SWIR emission as a reliable off-on fluorescence signal. This fluorophore design strategy of cooperation of ICT and ESIPT processes expands the in vivo sensing toolbox for accurate analysis in clinical applications.