文献类型：期刊文献

英文题名：A novel amide stationary phase for hydrophilic interaction liquid chromatography and ion chromatography

作者：Shen, Guobin[1];Zhang, FeiFang[1];Yang, Bingcheng[1];Chu, Changhu[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

年份：2013

卷号：115

起止页码：129

外文期刊名：TALANTA

收录：;EI(收录号：20132116359266);WOS:【SCI-EXPANDED(收录号:WOS:000328095600016)】；

基金：The work has been financially supported by National Science and Technology Major Science Instrument Project of the Ministry of Science and Technology of China (2012YQ090229) and National Natural Science Foundation of China (Nos. 21075038 and 21177040).

语种：英文

外文关键词：Hydrophilic interaction liquid; chromatography; Amide stationary phase; Ion chromatography; Click chemistry

摘要：A novel amide stationary phase (ASP) for hydrophilic interaction liquid chromatography (HILIC) has been prepared via the Click chemistry method. It was based on the strategy that the amino group of Asparagine was easily transferred to the corresponding azido group and then clicked onto terminal alkyne-silica gel in the presence of Cu(I)-based catalyst. For the tested polar compounds including nucleosides and nucleic acid bases, ASP-based column has demonstrated good performance in terms of separation efficiency and column stability, and the retention mechanism was found to match well the typical HILIC retention. In addition, the ASP described here showed much better selectivity in separation of inorganic anions under ion chromatography mode relative to other kinds of commercial ASP. (c) 2013 Elsevier B.V. All rights reserved.