文献类型：期刊文献

英文题名：Insight into aggregation-induced emission characteristics of red-emissive quinoline-malononitrile by cell tracking and real-time trypsin detection

作者：Shao, Andong[1,2];Guo, Zhiqian[1,2];Zhu, Shaojia[3];Zhu, Shiqin[1,2];Shi, Ping[3];Tian, He[1,2];Zhu, Weihong[1,2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：5

期号：4

起止页码：1383

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20141117453126);WOS:【SCI-EXPANDED(收录号:WOS:000332467400015)】；

基金：This work was supported by the National 973 Program (2013CB733700), NSFC/China, NSFC for Distinguished Young Scholars (Grant no. 21325625), the National Major Scientific Technological Special Project (2012YQ15008709), the Oriental Scholarship, the Fundamental Research Funds for the Central Universities (WK1013002, WJ1114013, 222201313010), SRFDP 20120074110002, and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：High resolution transmission electron microscopy - Solutions - Dynamic light scattering - Hydrophilicity

摘要：Water-soluble, long wavelength fluorescent aggregation-induced emission (AIE)-active materials are in great demand for high contrast biosensing and bioimaging. The substitution position effects of the sulfonate group on the basis of two quinoline-malononitrile (QM) derivatives (EDS and EDPS) provide insight into efficient modulation in the hydrophilicity, emitting color, and specific AIE characteristics. EDS shows a unique AIE behaviour in aqueous solution, but EDPS does not. The abnormal non-fluorescence aggregation for EDS in pure water is capsule-like with loose packing characteristics, but still has enough cavities or free volume to consume the radiative energy, resulting in nearly no fluorescence. When binding with the protein BSA, the sulfonate unit as a conformation function group (CFG) plays a vital role in altering its initial loose ensemble into tightly compact aggregation with light-up AIE characteristics. By cell tracking, dynamic light scattering (DLS) and transmission electron microscopy (TEM), the key role of sulfonate groups in the conformation alteration has been well demonstrated for the first time. Moreover, EDS is successfully exploited in a label-free real time AIE fluorescent assay for trypsin detection and inhibitor screening. The hydrophilic sulfonate group from the different substitution position in the AIE-active QM building blocks provides an effective way to tailor the intermolecular aggregation associated with molecular stacking, especially for in situ cell tracking and real-time trypsin detection.