文献类型：期刊文献

中文题名：接种密度对Vero细胞在微载体表面生长的影响

英文题名：Effect of the Seeding Density on the Growth of Vero Cells on Microcarriers

作者：孙祥明[1];谭文松[1];张元兴[1];华平[1];周亚竞[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1999

卷号：25

期号：4

起止页码：367

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2000045118547);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家"九五"重点科技攻关计划的资助

语种：中文

中文关键词：Vero细胞;细胞培养;接种密度;微载体表面生长

外文关键词：Vero cells; animal cell culture; seeding density; specific growth rate

摘要：研究了接种密度对Ｖｅｒｏ细胞在微载体表面生长行为的影响，发现培养过程中所获得的细胞最高密度随接种密度的增加而有所提高，当细胞的表面接种密度高于１．０×１０５ｃｅｌｓ／ｍｇＭＣ时，因贴壁后细胞扩展受到限制而不利于进入对数生长期。就整个Ｖｅｒｏ细胞培养过程，适宜的表面接种密度为３×１０４～７×１０４ｃｅｌｓ／ｍｇＭＣ，此时可以获得较高的比生长速率和细胞增殖倍数。实验结果还表明Ｖｅｒｏ细胞在ＣＴ－３微载体表面呈单层生长且不受表面限制，所达到的最高密度约为５．２×１０５ｃｅｌｓ／ｍｇＭＣ。
The effect of cell seeding density on the growth of Vero cells on microcarriers was investigated. It was found that the maximal cell density obtained in the culture process increased with increasing inoculum size. However, if the surface seeding density higher than 1.0105 cells/mgMC, the cell growth was hindered to enter the exponential growth phase, due to the limitation of cell extension after attachment. The experimental results showed that the suitable cell seeding density on microcarrier surfaces was in the range of 3104 to 7104 cells/mgMC, in which the specific growth rate and the cell expansion ratio would reach high. In addition, it was indicated that the maximal cell density on microcarriers CT3 as monolayers was about 5.2105 cells/mg MC.