文献类型：期刊文献

英文题名：Fine tuning the LightOn light-switchable transgene expression system

作者：Ma, Zhengcai[1];Du, Zengmin[1];Chen, Xianjun[1];Wang, Xue[1];Yang, Yi[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：440

期号：3

起止页码：419

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000326857500011)】；

基金：This work was supported by the NSFC (31225008, 31071260 and 31170815), Specialized Research Fund for the Doctoral Program of Higher Education (20100074110010), Shanghai Science and Technology Commission (12JC1402900), Dawn Program of Shanghai Education Commission (11SG31), State Key Laboratory of Bioreactor Engineering, the 111 Project (B07023) and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Gene expression; Light; Induction efficiency; Background expression

摘要：Spatiotemporal control of transgene expression in living cells provides new opportunities for the characterization of gene function in complex biological processes. We previously reported a synthetic, light-switchable transgene expression system called LightOn that can be used to control gene expression using blue light. In the present study, we modified the different promoter segments of the light switchable transcription factor GAWK) and the target gene, and assayed their effects on protein expression under dark or light conditions. The results showed that the LightOn system maintained its high on/off ratio under most modifications, but its induction efficiency and background gene expression level can be fine-tuned by modifying the core promoter, the UAS(G) sequence number, the length of the spacer between UASG and the core promoter of the target protein, and the expression level of the GAVPO transcription factor. Thus, the LightOn gene expression system can be adapted to a large range of applications according to the requirements of the background and the induced gene expression. (C) 2013 Elsevier Inc. All rights reserved.