文献类型：期刊文献

英文题名：Toxicological assessment and underlying mechanisms of tetrabromobisphenol A exposure on the soil nematode Caenorhabditis elegans

作者：Liu, Fuwen[1];Zhang, Ying[1];Zhang, Meng[1];Luo, Qishi[2];Cao, Xue[1];Cui, Changzheng[1];Lin, Kuangfei[1];Huang, Kai[1]

机构：[1]East China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Yonker Environm Protect Co Ltd, Branch Shanghai, Shanghai 200051, Peoples R China

年份：2020

卷号：242

外文期刊名：CHEMOSPHERE

收录：;EI(收录号：20194507627405);WOS:【SCI-EXPANDED(收录号:WOS:000509786600059)】；

基金：The authors have no conflicts of interest. The C. elegans including wild-type N2 and mutational strain VC40 [cyp-1.3A7(gk31)] were supplied by the Caenorhabditis Genetics Center. This research was supported by the National Key R&D Program of China (2018YFC1803304 and 2018YFC1800705), National Natural Science Foundation of China (51708223, 41301329), Shanghai Municipal Science and Technology Commission (17DZ1202304).

语种：英文

外文关键词：Caenorhabditis elegans; TBBPA; Neurotoxicity; Transcriptome; Cyp-13A7

摘要：The widespread use of tetrabromobisphenol A (TBBPA) in industries has resulted in its frequent detection in environmental matrices, and the mechanisms of its associated hazards need further investigation. In this study, the nematode Caenorhabditis elegans (C elegans) was exposed to environmentally relevant concentrations of TBBPA (0, 0.1, 1, 10, 100, 200 mu g/L) to determine its effects. At TBBPA concentrations above 1 mu g/L, the number of head thrashes, as the most sensitive physiological indicator, decreased significantly. Using the Illumina HiSeq (TM) 2000 sequencer, differentially expressed genes (DEGs) were determined, and 52 were down regulated and 105 were up regulated in the 200 mu g/L TBBPA treatment group versus the control group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database analysis demonstrated that dorso-ventral axis formation is related to neurotoxicity; metabolism of xenobiotics by Cytochrome P450 (CYP450) and glutathione-S-transferase (GST) was found to be the vital metabolic mechanisms and were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). GST was ascribed to the augmentation because mutations in cyp-13A7 were constrained under TBBPA exposure. Additionally, oxidative stress indicators accumulated in a dose-dependent relationship. These results will help understand the molecular basis for TBBPA-induced toxicity in C. elegans and open novel avenues for facilitating the exploration of more efficient strategies against TBBPA toxicity. (C) 2019 Elsevier Ltd. All rights reserved.