文献类型：期刊文献

英文题名：Adipose-derived stem cell/FGF19-loaded microfluidic hydrogel microspheres for synergistic restoration of critical ischemic limb

作者：Wang, Ruihan[1,2,3];Wang, Fangqian[4,5];Lu, Shan[1,2,3];Gao, Bin[1,2,3];Kan, Yuanqing[1,2,3];Yuan, Tong[1,2,3];Xu, Yisheng[6];Yuan, Chen[6];Guo, Daqiao[1,2,3];Fu, Weiguo[1,2,3,9];Yu, Xiaohua[4,5,8];Si, Yi[1,2,3,7]

机构：[1]Fudan Univ, Zhongshan Hosp, Dept Vasc Surg, Shanghai 200032, Peoples R China;[2]Fudan Univ, Inst Vasc Surg, Shanghai 200032, Peoples R China;[3]Natl Clin Res Ctr Intervent Med, Shanghai 200032, Peoples R China;[4]Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Orthoped, Hangzhou 310009, Zhejiang, Peoples R China;[5]Key Lab Motor Syst Dis Res & Precis Therapy Zhejia, Hangzhou 310000, Zhejiang, Peoples R China;[6]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[7]Fenglin Rd 180, Shanghai 200032, Peoples R China;[8]Jiefang Rd 88, Hangzhou, Zhejiang, Peoples R China;[9]Fenglin Rd 180, Shanghai, Peoples R China

年份：2023

卷号：27

起止页码：394

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000994797100001)】；

基金：Acknowledgements This work was financially supported by the National Natural Science Foundation of China (grant number 8207021027) , Shanghai Clinical Research Center for Interventional Medicine (grant number 19MC1910300) and Shanghai Science and Technology Commission (grant numbers 19441906600, 21S31904800) .

语种：英文

外文关键词：Microfluidic technology; Stem cell; Cytokine; Porous microspheres; Ischemic limb salvage

摘要：The efficacy of stem cell therapy is substantially compromised due to low cell survival rate and poor local retention post-delivery. These issues drastically limit the application of stem cells for ischemic limb therapy, which requires effective blood perfusion and skeletal muscle regeneration. Herein, based on microfluidic technology, an integrated stem cell and cytokine co-delivery system designed for functional ischemic limb salvage was constructed by first incorporating the myogenic cytokine, fibroblast growth factor 19 (FGF19), into microspheres composed of methacrylate gelatin (GelMA). Then adipose-derived stem cells (ADSCs) were highly absorbed into the porous structure of the microspheres, overcoming the insufficient loading efficiency and activities by conventional encapsulation strategy. The fabricated ADSCs/FGF19@mu sphere system demonstrated a uniform size of about 180 mu m and a highly porous structure with pore sizes between 20 and 40 mu m. The resultant system allowed high doses of ADSCs to be precisely engrafted in the lesion and to survive, and achieved sustained FGF19 release in the ischemic region to facilitate myoblast recruitment and differentiation and myofibrils growth. Furthermore, the combination of ADSCs and FGF19 exhibited a positive synergistic effect which substantially improved the therapeutic benefit of angiogenesis and myogenesis, both in vitro and in vivo. In summary, a stem cell and cytokine co-delivery system with the properties of easy preparation and minimal invasiveness was designed to ensure highly efficient cell delivery, sustained cytokine release, and ultimately realizes effective treatment of ischemic limb regeneration.