文献类型：期刊文献

英文题名：Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH₄⁺ concentration

作者：Zhang, Jianguo[1];Wang, Xuedong[1];Zheng, Yu[1];Fang, Guochen[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：31

期号：2

起止页码：63

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000252467200002)】；

语种：英文

外文关键词：S-adenosylmethionine; fed-batch fermentation; NH4+ concentration; Pichia pastoris

摘要：Yield of S-adenosylmethionine was improved significantly in recombinant Pichia pastoris by controlling NH4+ concentration. The highest production rate was 0.248 g/L h when NH4+ concentration was 450 mmol/L and no repression of cell growth was observed. Within very short induction time (47 h), 11.63 g/L SAM was obtained in a 3.7 L bioreactor.