文献类型：期刊文献

英文题名：Beta-ionone is a functional plant volatile that attracts the parasitic wasp, Microplitis pallidipes

作者：Zhang, Hao[1];Wang, Jin-Yan[1];Chen, Yi-Juan[1];Siemann, Evan[2];Ji, Xiang-Yun[1];Jiang, Jie-Xian[1];Wan, Nian-Feng[1,3]

机构：[1]Shanghai Acad Agr Sci, Ecoenvironm Protect Inst, Shanghai Engn Res Ctr Low Carbon Agr, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China;[2]Rice Univ, Dept Biosci, Houston, TX 77005 USA;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Ecol Entomol Grp, Shanghai 200237, Peoples R China

年份：2022

卷号：67

期号：1

起止页码：1

外文期刊名：BIOCONTROL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000698030700001)】；

基金：This work was supported by the Shanghai Agriculture Applied Technology Development Program (Grant No. T20210204), National Natural Science Foundation of China (Grant No. 31601641, 32001969), Natural Science Foundation of Shanghai (Grant No. 20ZR1449000), Shanghai Agriculture Commission of China (2014-7-3-2) and SAAS Program for Excellent Research Team (2018[B-01]).

语种：英文

外文关键词：Biological control; Field screenhouse experiments; Fluorescence competitive binding assay; Odorant binding protein; Parasitoid; Y-tube olfactometer

摘要：The effects of plant volatiles on parasitoids are important with regards to the tri-trophic interactions among host plants, insect herbivores, and their natural enemies. However, the effects of plant volatiles on the important parasitoid (Microplitis pallidipes Szepligeti; Hymenoptera: Braconidae) of noctuids have not been determined at the molecular, laboratory, and outdoor scales with complementary approaches. The odorant binding protein 8 of M. pallidipes (MpOBP8) with an open reading frame of 459 bp was cloned and successfully expressed in Escherichia coli (Eubacteriales: Enterobacteriaceae). The purified protein MpOBP8 had a relatively high binding capacity to the plant volatile beta-ionone (Ki value was 28.86 mu M) in the fluorescence competitive binding assay. Meanwhile, Y-tube olfactometer experiments indicated that beta-ionone selectively attracts the parasitic wasp as the ratio of M. pallidipes selecting beta-ionone vs. control was 2:1. Likewise, field screenhouse experiments verified the above results as the ratio of M. pallidipes selecting beta-ionone vs. control was close to 2:1. This study suggested that beta-ionone is a plant volatile that attracts the parasitoid M. pallidipes, and MpOBP8 may play a key role in the discrimination of M. pallidipes in searching for hosts via beta-ionone. In general our results suggest that beta-ionone has the potential to enhance the biocontrol effect of parasitoids on insect herbivores.