文献类型：期刊文献

英文题名：Metabolic engineering of Escherichia coli for the biosynthesis of α-copaene from glucose

作者：Zhang, Suping[1];Zhao, Xin[1];He, Xiyuan[1];Yang, Liu[1];Wang, Yilin[1];Liu, Feng[2];Wang, Chao[1];Hua, Qiang[2];Wu, Yongchao[3];Liu, Zhijie[1]

机构：[1]Hubei Univ Technol, Cooperat Innovat Ctr Ind Fermentat Minist Educ &, Natl 111 Ctr Cellular Regulat & Mol Pharmaceut, Key Lab Fermentat Engn,Minist Engn,Hubei Key Lab, Wuhan 430068, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Hubei Juhui Agr Co Ltd, Jingmen 431821, Peoples R China

年份：2022

卷号：186

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20223212552639);WOS:【SCI-EXPANDED(收录号:WOS:000880816000003)】；

基金：This work was financially supported by National Natural Science Foundation of China (22007031) and Collaborative Grant-in-Aid of the HBUT National "111'' Center for Cellular Regulation and Molecular Pharmaceutics (XBTK-2022012).

语种：英文

外文关键词：Escherichia coli; alpha-copaene; MVA pathway; Metabolic engineering

摘要：alpha-Copaene, a tricyclic sesquiterpene, has broad application prospects in many fields, including food, medicine, and agriculture. The conventional plant extraction method of alpha-copaene is unsustainable. In this study, for the first time, the de novo biosynthesis of alpha-copaene from glucose was achieved using a metabolically engineered Escherichia coli strain. alpha-Copaene was successfully produced in the engineered strain that heterologously expressed Piper nigrum alpha-copaene synthase. A series of strategies were employed to enhance alpha-copaene biosynthesis by E. coli, including heterologous overexpression of Saccharomyces cerevisiae farnesyl diphosphate synthase, MVA pathway, and truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase 1. Strain optimization led to a 78.1-fold increase in alpha-copaene level in E. coli. This study reports a sustainable method for alpha-copaene production and provides a metabolic engineering strategy for the biosynthesis of other sesquiterpenes in E. coli.