文献类型：期刊文献

英文题名：Deep Eutectic Solvent-Based Microwave-Assisted Extraction for the Chromatographic Analysis of Bioactive Flavonoids in Spirodela polyrrhiza

作者：Hao, Haitian[1];Lin, Long[1];Liu, Shun[1];Kang, Yun[2];Wang, Yaqin[2];Huang, Jianming[2];Weng, Weiyu[1,3]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai 200237, Peoples R China;[2]Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2022

卷号：60

期号：5

起止页码：501

外文期刊名：JOURNAL OF CHROMATOGRAPHIC SCIENCE

收录：;EI(收录号：20242616391419);WOS:【SCI-EXPANDED(收录号:WOS:000767666800001)】；

语种：英文

外文关键词：Chlorine compounds - Chromatographic analysis - Eutectics - High performance liquid chromatography - Solvent extraction - Solvents

摘要：Deep eutectic solvents (DESs) are regarded as promising solvents to extract chemicals from plant materials. In this study, a DES-based microwave-assisted extraction (MAE) method was developed for the chromatographic analysis of four bioactive flavonoids in Spirodela polyrrhiza. Chromatographic separation was achieved on a Promosil C-18-column. Prior to the HPLC analysis, the flavonoids were rapidly extracted by a DES-MAE process using choline chloride/levulinic acid (1:2, mol/mol) as the solvent. The extraction parameters were optimized using response surface methodology, and the optimal DES-MAE was fast and efficient compared with conventional solvent-based MAE and ultrasonic-assisted extraction using DES. The recoveries of optimized DES-MAE for the four flavonoids ranged from 97.80 to 103.29%. This study demonstrates that the validated DES-MAE-HPLC method is efficient, accurate and applicable to the determination of flavonoids in S. polyrrhiza.