文献类型：期刊文献

英文题名：Cr(VI) Reduction and Fe(II) Regeneration by Penicillium oxalicum SL2-Enhanced Nanoscale Zero-Valent Iron

作者：Luo, Yating[1];Pang, Jingli[1];Peng, Cheng[2];Ye, Jien[3];Long, Bibo[4];Tong, Jianhao[1];Shi, Jiyan[1]

机构：[1]Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou 310058, Peoples R China;[2]East China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asses, Shanghai 200237, Peoples R China;[3]Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo 315100, Peoples R China;[4]Guangdong Acad Sci, Inst Biol & Med Engn, Guangzhou 510316, Peoples R China

年份：2023

卷号：57

期号：30

起止页码：11313

外文期刊名：ENVIRONMENTAL SCIENCE & TECHNOLOGY

收录：;EI(收录号：20233214484994);WOS:【SCI-EXPANDED(收录号:WOS:001031756000001)】；

基金：This research was supported by the National Natural Science Foundation of China (42277004 and 41721001) and the National Key Research and Development Program of China (2022YFC3702104).

语种：英文

外文关键词：Cr(VI) reduction; combined remediation; nZVIactivation; RNA sequencing

摘要：Aggregationand passivation of nZVI limits applicationsin Cr(VI) remediation. This study developed an nZVI-activated compositesystem, P. oxalicum SL2-nZVI,and investigated the underlying interaction mechanisms. Nanoscale zero-valent iron (nZVI) faces significant challengesin Cr(VI) remediation through aggregation and passivation. This studyidentified a Cr(VI)-resistant filamentous fungus (Penicilliumoxalicum SL2) for nZVI activation and elucidated thesynergistic mechanism in chromium remediation. P. oxalicum SL2 and nZVI synergistically and effectively removed Cr(VI), mainlyby extracellular nonenzymatic reduction (89.1%). P.oxalicum SL2 exhibited marked iron precipitate solubilizationand Fe(II) regeneration capabilities. The existence of the Fe(II)-Cr(V)-oxalatecomplex (HCrFeC4O9) indicated that in additionto directly reducing Cr(VI), iron ions generated by nZVI stimulatedCr(VI) reduction by organic acids secreted by P. oxalicum SL2. RNA sequencing and bioinformatics analysis revealed that P. oxalicum SL2 inhibited phosphate transport channelsto suppress Cr(VI) transport, facilitated iron and siderophore transportto store Fe, activated the glyoxylate cycle to survive harsh environments,and enhanced organic acid and riboflavin secretion to reduce Cr(VI).Cr(VI) exposure also stimulated the antioxidative system, promotingcatalase activity and maintaining the intracellular thiol/disulfidebalance. Cr(VI)/Fe(III) reductases played crucial roles in the intracellularreduction of chromium and iron, while nZVI decreased cellular oxidativestress and alleviated Cr(VI) toxicity to P. oxalicum SL2. Overall, the P. oxalicum SL2-nZVIsynergistic system is a promising approach for regenerating Fe(II)while reducing Cr(VI).