文献类型：期刊文献

英文题名：Hydra gasdermin-gated pyroptosis signalling regulates tissue regeneration

作者：Chen, Shouwen[1];Gong, Yuxin[1];Li, Shuxin[1];Yang, Dahai[1];Zhang, Yuanxing[2,3];Liu, Qin[1,2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Marine Cultured Anim Vaccine, Shanghai 200237, Peoples R China

年份：2023

卷号：149

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001067666300001)】；

基金：We thank XB Hu, JM Xu, SL Gu, YZ Chen for their assistance with microscope and W Wu, JM Xu for their assistance with Hydra experiment. This work was supported by the NSFC (No. 32002429) to S.C., the China Postdoctoral Science Foundation (No. 2020M671033) to S.C., and The Fundamental Research Funds for the Central Universities (No. 222201231721) to Q.L.

语种：英文

外文关键词：Gasdermin; Pyroptosis; Wnt; Mitochondrial ROS; Regeneration

摘要：Pyroptosis, an inflammatory form of programmed cell death, is directly executed by gasdermin (GSDM) depending on its N-terminal pore-forming fragment-mediated membrane-disrupting, triggering intracellular contents release, which plays important roles in mammalian anti-infection and anti-tumor immune responses. However, whether pyroptosis engages in the regulation of tissue regeneration remains largely unknown. Here, utilizing Hydra vulgaris as the research model, we found that an HyCARD2-HyGSDME-mediated pyroptosis signalling is activated in both head and foot regenerated tips after amputation. Impeding pyroptosis by knocking down the expression of either HyGSDME or HyCARD2 significantly hampered both head and foot regeneration in Hydra. Mechanistically, the activation of HyCARD2-HyGSDME axis at wound sites is dependent of intracellular mitochondrial reactive oxygen species (mtROS), the removing of which hindered Hydra head regeneration. Moreover, the HyCARD2-HyGSDME axis-gated pyroptosis was found to enhance the initial secretion and upregulated expression of Wnt3. Collectively, these findings indicate that gasdermin-gated pyroptosis is critical for the evoking of Wnt signalling to facilitate Hydra tissue regeneration, which provides insights into functional diversification within the gasdermin family in the animal kingdom.