文献类型：期刊文献

英文题名：Evaluation of surfactant effect on β-poly(L-malic acid) production by Aureobasidium pullulans

作者：Yin, Haisong[1,2,3];Gao, Cui[1,4];Ye, Kai[1,4];Zhao, Tingbin[5];Sun, Aiyou[6];Qiao, Changsheng[1,2,4]

机构：[1]Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China;[2]Tianjin Modern Vocat Technol Coll, Sch Bioengn, Tianjin, Peoples R China;[3]Minist Educ, Engn Res Ctr Food Biotechnol, Tianjin, Peoples R China;[4]Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Engn Res Ctr Microbial Metab & Fermentat, Tianjin, Peoples R China;[5]Tianjin Huizhi Biotrans Bioengn Co Ltd, Tianjin, Peoples R China;[6]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2019

卷号：33

期号：1

起止页码：954

外文期刊名：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

收录：;EI(收录号：20194907772525);WOS:【SCI-EXPANDED(收录号:WOS:000472515200001)】；

基金：This work was supported by the Foundation of Tianjin Municipal Science and Technology Commission, PR China under grant numbers 17PTGCCX00190, 17PTSYJC00080, 17YFCZZC00310 and 16YFXTSF00460, the Foundation of Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, PR China under grant number ZXKF20180301 and the Foundation of Engineering Research Center of Food Biotechnology, Ministry of Education, PR China under grant number SPZX10-18.

语种：英文

外文关键词：Aureobasidium pullulans; poly (beta-L-malic acid); Tween 80; mechanism

摘要：beta-poly(L-malic acid) (PMLA) is a highly water-soluble biopolymer used in medicine and other industries. PMLA is usually biosynthesized by Aureobasidium pullulans, but in low concentrations, which has restricted its further large-scale production. Here effects of surfactants on PMLA production were investigated. Of various surfactants examined, the addition of Tween 80 at the start of cell growth increased mycelial growth, PMLA production, PMLA productivity and the ratio of PMLA to cell biomass concentration (Y-p/x) of A. pullulans. Transmission electron microscopy and flow cytometry showed that cell membrane permeability was increased, improving the secretion of PMLA and uptake of nutrients and oxygen. Proteomic analysis showed that key pathways of PMLA synthesis, along with cellular energy metabolism, were enhanced. Moreover, glutathione (GSH) synthesis and anti-oxidation capacity were also improved. These results indicated that the addition of Tween 80 is an efficient approach for improving the production of PMLA.