文献类型：期刊文献

英文题名：An electroconductive hydrogel with injectable and self-healing properties accelerates peripheral nerve regeneration and motor functional recovery

作者：Yi, Zhenwei[1,3];Zhan, Fangke[1,3];Chen, Yijia[1,3];Zhang, Ran[1,3];Lin, Haodong[2];Zhao, Liming[1,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Med, Shanghai Gen Hosp, Dept Orthopaed, Shanghai 200080, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2023

卷号：478

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20234715091045);WOS:【SCI-EXPANDED(收录号:WOS:001116542700001)】；

基金：This work was supported by the Program of Shanghai Academic/Technology Research Leader (22XD1430500) and the Programme of Introducing Talents of Discipline to Universities (No. B18022) .

语种：英文

外文关键词：Electroconductive hydrogel; Injectability; Self -healing; Peripheral nerve regeneration

摘要：Peripheral nerve regeneration and functional recovery are slow due to disruption of bioelectrical signal trans-mission. As a new therapeutic strategy, conductive hydrogels have been shown to promote the regeneration of electroactive tissue, especially nerve tissue. However, conventional conductive hydrogels made of physically doped conductive particles suffer from fragility, agglomeration, poor durability, and discontinuous transmission of electrical signals, severely limiting the migration of Schwann cells (SCs) and the remyelination of axons. Herein, a novel water-soluble conductive material was synthesized by grafting polyaniline (PANI) onto carboxymethyl chitosan (CMCS). Dual cross-linked hydrogels with injectable, self-healing, and conductive properties were created by adding CMCS-PANI (CP) to the dynamic gel network generated by the Schiff base reaction between aldehyde-based hyaluronic acid (ALHA) and CMCS. The prepared self-healing ALHA/CMCS/CP (ACCP) hydrogel had good biocompatibility, elastic modulus, and electrical conductivity that matched the sciatic nerve. The in vitro experiments indicated that ACCP3 (3 wt% CP) promoted the proliferation and migration of the SCs. Injection of ACCP3 into the crush injury site of the sciatic nerve reduced tissue resistivity, increased nerve conduction velocity, decreased the sciatic nerve functional index, enhanced the expression of neuronal axon -specific proteins, induced axon extension and remyelination, and prevented muscle denervation atrophy. Overall, the injectable self-healing conductive ACCP3 hydrogel, as a safe and effective neural tissue substitute, opens up a new way for the treatment of peripheral nerve injury and has potential clinical application.