文献类型：期刊文献

英文题名：Dual-functionalized magnetic bimetallic metal-organic framework composite for highly specific enrichments of phosphopeptides and glycopeptides

作者：Pan, Yini[1];Zhang, Chenhui[1];Xiao, Ruili[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai, Peoples R China

年份：2021

卷号：1158

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20211310155883);WOS:【SCI-EXPANDED(收录号:WOS:000635222200001)】；

基金：We gratefully acknowledged the support of the National Natural Science Foundation of China (No.21974045), the Science and Technology Commission of Shanghai Municipality (No.19142201100, 6142200500), the Fundamental Research Funds for the Central Universities (No.50321102117022).

语种：英文

外文关键词：Bimetallic metal-organic framework; Dual-function; Simultaneously enrichment; Phosphopeptides; Glycopeptides

摘要：In this work, a dual-functionalized magnetic bimetallic metal-organic framework composite denoted as Fe3O4@SiO2@(Zr-Ti-MOF)(10)-NH2 was ingeniously designed and fabricated by a facial layer-by-layer assembly technique. The composite not only exhibited strong affinity for phosphopeptide due to the coexistence of Zr-O clusters and Ti-O clusters, but also owned great hydrophilicity for glycopeptides relying on abundant hydrophilic NH2 groups, meeting the demand for simultaneously enrichment and sequential elution of phosphopeptides and glycopeptides. As expected, the synthesized composite showed great selectivity (1:2000 M ratio of beta-casein:BSA; 1:50 M ratio of IgG:BSA), good sensitivity (1 fmol mu L-1 for both alpha-casein and IgG), and good capacity (80 mg g(-1) for alpha-casein and 200 mg g(-1) for IgG). By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from alpha-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. Furthermore, the composite was successfully applied to the analysis of intricate biological samples. 4 endogenous phosphopeptides and 20 phosphopeptides were trapped from human serum and non-fat milk tryptic digest respectively. From 0.5 mg of tryptic digest of rat brain, 141 N-linked glycopeptides corresponding to 127 glycoproteins and 918 phosphopeptides corresponding to 397 phosphoproteins were enriched simultaneously and identified respectively, proving the Fe3O4@SiO2@(Zr-Ti-MOR10-NH2 to be a dependable candidate for the simultaneously enrichment of trace phosphopeptides and glycopeptides in intricate biological samples. (C) 2021 Elsevier B.V. All rights reserved.