文献类型：期刊文献

英文题名：Simultaneous Two-Color Visualization of Lipid Droplets and Endoplasmic Reticulum and Their Interplay by Single Fluorescent Probes in Lambda Mode

作者：Guo, Lifang[1,2,3,4];Tian, Minggang[1];Zhang, Zhiyun[2,3];Lu, Qing[1];Liu, Zhiqiang[1];Niu, Guangle[1,4];Yu, Xiaoqiang[1,4]

机构：[1]Shandong Univ, Adv Med Res Inst, State Key Lab Crystal Mat, Jinan 250100, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Shandong Univ, Shenzhen Res Inst, Shenzhen 518057, Peoples R China

年份：2021

卷号：143

期号：8

起止页码：3169

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20210909984171);WOS:【SCI-EXPANDED(收录号:WOS:000626325000020)】；

基金：Our laser confocal scanning imaging of living cells was performed at The Microscopy Characterization Facility, Shandong University. The study was financially supported by the National Natural Science Foundation of China (52073163, 51773111, 21672130, 51273107 and 22004036), the Natural Science Foundation of Shandong Province, China (ZR2017ZC0227), the Fundamental Research Funds of Shandong University (2017JC011 and 2018JC030), the Special Fund of Taishan Scholars Project of Shandong Province, China (tsqn201909012), the Guangdong Basic and Applied Basic Research Foundation (2020A1515110578), the Opening Fund of Key Laboratory of Photochemical Conversion and Optoelectronic Materials, TIPC, CAS (PCOM202001), and the Program of Qilu Young Scholars of Shandong University. We also sincerely thank Prof. Dongfeng Xue at Shandong University for providing valuable advices and discussions to further improve the quality of this work.

语种：英文

外文关键词：Color - Visualization - Drops - Excited states - Cell proliferation - Fluorescence imaging

摘要：In living systems, subcellular organelles mutually cooperate and closely contact to form organelle interaction networks. Thus, the simultaneous and discriminative visualization of different organelles is extremely valuable for elucidating their distribution and interplay. However, such meaningful investigations remain a great challenge due to the lack of advanced single fluorescent probes (SF-probes) capable of simultaneous and two-color imaging of two targets. Herein, for the first time, we present two excited-state intramolecular proton transfer (ESIPT) based SF-probes (PPC and EPC) for simultaneous two-color fluorescence imaging of lipid droplets (LDs) and the endoplasmic reticulum (ER) under single-wavelength excitation. Due to the strong electron-donating ability of the side substituents, the fluorescence spectra and colors of these ESIPT probes are highly sensitive to the nuance of water contents between LDs and ER, leading to orange and green fluorescence in LDs and ER, respectively, in the Lambda imaging mode. Using the probe PPC or EPC, the morphology, size, and distribution of LDs and ER have been investigated in live cells and tissues. With the aid of in situ and real-time fluorescence imaging in Lambda mode, we observed the generation of newborn LDs near the ER regions and their close apposition and shared identical fluorescence colors, probably providing a valuable proof for the mainstream hypothesis that LDs originate from the ER. The remarkable imaging performances render these SF-probes as powerful tools to decipher LD-ER related biological processes.