文献类型：期刊文献

中文题名：红球菌Rhodococcus.sp G14生物转化合成2-苯基丙酸

英文题名：Production of 2-phenylpropionic Acid by Rhodococcus sp. G14

作者：左可[1];李志敏[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2013

卷号：29

期号：12

起止页码：173

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

语种：中文

中文关键词：生物催化;异丙苯;2-苯基丙酸;优化

外文关键词：Biotransformation Cumene 2-phenylpropionic acid Optimization

摘要：2-苯基丙酸作为布洛芬合成过程中的一种重要的医药中间体,目前对其的生产及应用正在逐年增加,但价格十分昂贵。对野生红球菌Rhodococcussp.G14生物催化异丙苯合成2-苯基丙酸的条件进行探索。利用响应面优化方法,得到菌体生长最适培养基,分批培养OD600可达到19.82。单因素试验优化菌体反应时间、反应温度、反应菌浓、底物浓度、渗透处理,获得最佳催化条件下2-苯基丙酸产量为14.30 mg/L,为后续优化提高打下了基础。
As a kind of important medicine intermediate in ibuprofen synthesis, the demand and application of 2-phenylpropionic acid（2-PPA）is increased year by year. In this study, the reaction conditions for the biotransformation of cumune to 2-PPA by wild type Rhodococcus sp. G14 was investigated. Using response surface optimization, the best medium condition for growth which could result 19.82 of OD600 in batch cultivation was obtained. After single factor experiment on reaction time, reaction temperature, OD600, concentration of substrate, osmotic treatment, 14.30 mg/L of 2-PPA was obtained, which would benefit the subsequent optimization.