文献类型：期刊文献

英文题名：Chemical characteristics and hemostatic performances of ordered mesoporous calcium-doped silica xerogels

作者：Wu, Xiaohui;Wei, Jie;Lu, Xun;Lv, Yang;Chen, Fangping;Zhang, Yanglan;Liu, Changsheng[1]

机构：[1]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2010

卷号：5

期号：3

外文期刊名：BIOMEDICAL MATERIALS

收录：;EI(收录号：20102513017701);WOS:【SCI-EXPANDED(收录号:WOS:000278408500006)】；

基金：The authors are indebted to the financial support from the Major Program of National Natural Science Foundation of China (no 50732002), the National Natural Science Funds of China (no 30970720), Nano special program of Science and Technology Development of Shanghai (no 0852nm02700), Standard Special program of Science and Technology Development of Shanghai (no 08DZ0501600).

语种：英文

外文关键词：Apatite - Silica gel - Blood - Sol-gel process - Synthesis (chemical) - Xerogels - Biocompatibility - Bioactivity - Calcium

摘要：A well-ordered mesoporous silicon dioxide calcium-doped xerogel (m-SXC) was synthesized by a sol-gel process. The in vitro bioactivity of the m-SXC was investigated by analyzing the apatite-forming ability in simulated body fluid (SBF) and MC3T3-E1 cell culture with materials, and the hemostatic activity of the m-SXC was determined by measuring the activated partial thromboplastin time (APTT) and prothrombin time (PT) in vitro. The results suggested that the m-SXC exhibited excellent in vitro bioactivity, with surface apatite formation for the m-SXC containing 10 w% Ca (m-SXC10) exceeding that containing 5 w% Ca (n-SXC5) and 0% Ca (m-SXC0) at 7 days, and the cell response test (MTT assay) and cell morphology observation demonstrated that these materials were biocompatible with no obvious negative effects on cellular viability. Moreover, the m-SXC with a high surface area (m-SXC0: 489.9 m(2) g(-1), m-SXC5: 466.5 m(2) g(-1), m-SXC10: 438.1 m(2) g(-1)) possessed good hemostatic property because it could absorb a large amount of water from the blood, which could concentrate the components of blood and reduce the clotting time. Furthermore, the addition of calcium in the m-SXC had significant effects on the clotting time, in which the APTT (m-SXC0: 8 s, m-SXC5: 7 s, m-SXC10: 6 s, respectively) and PT (m-SXC0: 16 s, m-SXC5: 11 s, m-SXC10: 10 s, respectively) were shortened with the increase of Ca amounts in the m-SXC, indicating that incorporation of Ca in the m-SXC could improve its hemostatic activity.