文献类型：期刊文献

中文题名：LC-MS方法分析皮肤表面鞘脂的组成

英文题名：Identification of Sphingolipid Compositions in the Skin by LC-MS

作者：佟薇[1];江芝婷[2];胡坪[1];章弘扬[1]

机构：[1]华东理工大学化学与分子工程学院上海市功能性材料化学重点实验室,上海200237;[2]巴斯夫新材料有限公司个人护理品部,上海200137

年份：2022

卷号：44

期号：9

起止页码：1368

中文期刊名：化学试剂

外文期刊名：Chemical Reagents

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：国家自然科学基金资助项目(81973285)。

语种：中文

中文关键词：皮肤表面鞘脂;UPLC-Q-TOF-MS;多元变量统计分析;皮肤老化;差异性标志物

外文关键词：skin surface sphingolipids;UPLC-Q-TOF-MS;multivariate statistical analysis;skin aging;differential markers

摘要：鞘脂作为角质层中最丰富的脂质,在皮肤屏障中起到重要作用。通过建立一种基于超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)的分析方法,利用自建数据库匹配和二级质谱结构解析,对女性皮肤表面的108个鞘脂进行了鉴定并归纳其碎裂机理,其中包括34个鞘氨醇碱、65个神经酰胺(Cer)、8个1-磷酸神经酰胺(CerP)和1个磷酰鞘脂(PN-Cer)。随后将该方法应用于青、中年女性前臂皮肤鞘脂的检测,结合多元变量统计分析,初步探索了皮肤老化的鞘脂代谢差异。结果表明,不同年龄女性皮肤的鞘脂轮廓表现出明显的分组趋势,并筛选得到43个差异性鞘脂标志物,这些标志物相对含量的变化可能与衰老皮肤细胞中酶活性的变化有关。为皮肤鞘脂的全面分析提供了新方法,也对皮肤衰老机制的探索及相关抗衰老护肤品的研发具有一定的理论指导意义。
Sphingolipids,as the most abundant lipids in the stratum corneum,play an important role in skin barrier.An analytical method based on ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was established to analyze the sphingolipids in women skin surface.By using personal database matching and MS/MS structural analysis,108 sphingolipids were identified in the skin of women,including 34 of sphingosine bases,65 of ceramides(Cer),8 of ceramide 1-phosphates(CerP),and 1 of phosphonosphingolipid(PN-Cer).After then,this method combined with multivariate statistical analysis was applied to detect the metabolic perturbations of sphingolipids associated with skin aging in the young and middle-aged women forearm.The results showed that the skin sphingolipid profiles of young and middle-aged women exhibiting an obvious grouping trend,and 43 of differentiated sphingolipid markers were screened,which might be related to the changes of enzyme activities in aging skin cells.This study could provide a new method for the comprehensive analysis of skin surface sphingolipids,and it also has certain theoretical guiding significance for the exploration of skin aging mechanism and the research and development of related anti-aging skin care products.