文献类型：期刊文献

英文题名：RhBMP-2-loaded calcium silicate/calcium phosphate cement scaffold with hierarchically porous structure for enhanced bone tissue regeneration

作者：Zhang, Jing[1,2,3];Zhou, Huanjun[1,2];Yang, Kai[2,3];Yuan, Yuan[1,2,3];Liu, Changsheng[1,2,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2013

卷号：34

期号：37

起止页码：9381

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20134016819280);WOS:【SCI-EXPANDED(收录号:WOS:000326901200015)】；

基金：The authors wish to express their gratitude to the financial supports from National Basic Research Program of China (973 Program, No. 2012CB933600), National Natural Science Foundation of China (No. 31100679), National Science and Technology Support Program (2012BAI17B02), and Shanghai Nanotechnology Special Foundation (No. 11nm0506300). This study was also supported by Program for New Century Excellent Talents in University.

语种：英文

外文关键词：Calcium silicate/calcium phosphate cement; rhBMP-2; Bone regeneration; Hierarchical porosity

摘要：Calcium phosphate cement scaffold (CPC) has been widely used as bone graft substitutes, but undesirable osteoinductivity and slow degradability greatly hamper their clinic application. To address these problems, a recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded calcium silicate/calcium phosphate cement scaffold (CSPC) with hierarchical pores was developed in this study. The CSPC scaffold with both interconnected macropores on the order of 200-500 mu m and micropores of 2-5 mu m was synthesized from CPC and calcium silicate (CS) by a NaCl particulate-leaching method. In vitro cell culture with C2C12 model cells, in vivo ectopic bone formation and rabbit femur cavity defect repair were performed to evaluate the osteogeneic capacity of the CSPC/rhBMP-2 scaffold. CPC, CSPC and CPC/rhBMP-2 scaffolds were parallelly investigated for comparison. The results demonstrated that the hierarchical macro/microporous structure, whether in presence of CS or rhBMP-2, highly favored the adhesion of C2C12 cells and bone in-growth into the CPC-based scaffolds. But, in comparison to the CPC-based scaffolds with CS or rhBMP-2 alone, the CSPC/rhBMP-2 scaffold strongly promoted osteogenic differentiation in vitro and osteogenetic efficacy in vivo. Further studies demonstrated that Si ions derived from CSPC contributed mainly to maintain the conformation of rhBMP-2 and thus stimulate the synergistic action of CS and rhBMP-2 in osteogenic differentiation and osteoinductivity. Additionally, the incorporation of CS was also beneficial for the dissolution of the scaffold. Those results suggest that the CSPC has superior properties for incorporation of rhBMP-2 and our developed CSPC/rhBMP-2 scaffold have great potential for future use in bone tissue regeneration. (C) 2013 Elsevier Ltd. All rights reserved.