文献类型：期刊文献

英文题名：The insecticide spinosad induces DNA damage and apoptosis in HEK293 and HepG2 cells

作者：Yang, Mingjun[1];Xiang, Guanggang[1];Li, Diqiu[1];Zhang, Yang[1];Xu, Wenping[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2016

卷号：812

起止页码：12

外文期刊名：MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000390511200002)】；

基金：This work was financially supported by the National Key Technology Research Development Program of China (NO. 2011BAE06B04).

语种：英文

外文关键词：Spinosad; DNA damage; Comet assay; H2AX foci; Apoptosis; Cytotoxic

摘要：Spinosad, a pesticide acting on the central nervous system of insects, is classified as a pesticide with reduced risk. However, spinosad-induced toxicological effects on non-target organisms must not be ignored. This study aimed to evaluate the cytotoxicity and potential genotoxicity of spinosad in HEK293 and HepG2 cell lines. The results showed that spinosad caused a concentration- and time-dependent decrease in cell viability of HEK293 and HepG2 cells. Spinosad-induced p53 accumulation thereby upregulates the expression of Bax and downregulates the expression of Bcl-2. Further studies confirmed that spinosad induced apoptosis in HEK293 and HepG2 cells, accompanied by a dissipation of the mitochondria) membrane potential and an increase in caspase-3 activity. The alkaline comet assay and,gamma-H2AX foci staining revealed that spinosad induced significant concentration-dependent increases of DNA strand breaks in HEK293 and HepG2 cells. Our results indicate that spinosad effectively induced DNA damage and apoptosis in HEK293 and HepG2 cells. (C) 2016 Elsevier B.V. All rights reserved.