文献类型：期刊文献

英文题名：Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity

作者：Ni, Yan[1];Li, Chun-Xiu[1];Ma, Hong-Min[1];Zhang, Jie[1];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Bioproc, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：89

期号：4

起止页码：1111

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20111113747585);WOS:【SCI-EXPANDED(收录号:WOS:000287143600024)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 20773038 and 20902023), Ministry of Science and Technology, P.R. China (Nos. 2009CB724706 and 2009ZX09501-016), China National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and Shanghai Leading Academic Discipline Project (No. B505).

语种：英文

外文关键词：YtbE; Recombinant aldo-keto reductase; Bacillus sp.; Asymmetric reduction; Chiral alcohols

摘要：In the screening of 11 E. coli strains overexpressing recombinant oxidoreductases from Bacillus sp. ECU0013, an NADPH-dependent aldo-keto reductase (YtbE) was identified with capability of producing chiral alcohols. The protein (YtbE) was overexpressed, purified to homogeneity, and characterized of biocatalytic properties. The purified enzyme exhibited the highest activity at 50A degrees C and optimal pH at 6.5. YtbE served as a versatile reductase showing a broad substrate spectrum towards different aromatic ketones and keto esters. Furthermore, a variety of carbonyl substrates were asymmetrically reduced by the purified enzyme with an additionally coupled NADPH regeneration system. The reduction system exhibited excellent enantioselectivity (> 99% ee) in the reduction of all the aromatic ketones and high to moderate enantioselectivity in the reduction of alpha- and beta-keto esters. Among the ketones tested, ethyl 4,4,4-trifluoroacetoacetate was found to be reduced to ethyl (R)-4,4,4-trifluoro-3-hydroxy butanoate, an important pharmaceutical intermediate, in excellent optical purity. To the best of our knowledge, this is the first report of ytbE gene-encoding recombinant aldo-keto reductase from Bacillus sp. used as biocatalyst for stereoselective reduction of carbonyl compounds. This study provides a useful guidance for further application of this enzyme in the asymmetric synthesis of chiral alcohol enantiomers.