文献类型：期刊文献

英文题名：Fluorescence Probes Exhibit Photoinduced Structural Planarization: Sensing In Vitro and In Vivo Microscopic Dynamics of Viscosity Free from Polarity Interference

作者：Wu, Cheng-Ham[1];Chen, Yi[1,4,5];Pyrshev, Kyrylo A.[2,6];Chen, Yi-Ting[1];Zhang, Zhiyun[1,4,5];Chang, Kai-Hsin[1];Yesylevskyy, Semen O.[7,8];Demchenko, Alexander P.[2,3];Chou, Pi-Tai[1]

机构：[1]Natl Taiwan Univ, Dept Chem, Taipei 10607, Taiwan;[2]Natl Acad Sci Ukraine, Palladin Inst Biochem, UA-01030 Kiev, Ukraine;[3]Yuriy Fedkovych Natl Univ, UA-58012 Chernovtsy, Ukraine;[4]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[6]Natl Acad Sci Ukraine, Inst Phys, UA-01030 Kiev, Ukraine;[7]Univ Bourgogne Franche Comte, UMR CNRS 6249, Lab Chrono Environm, F-25030 Besancon, France;[8]Natl Acad Sci Ukraine, Inst Phys, UA-03028 Kiev, Ukraine

年份：2020

卷号：15

期号：7

起止页码：1862

外文期刊名：ACS CHEMICAL BIOLOGY

收录：;EI(收录号：20210152290);WOS:【SCI-EXPANDED(收录号:WOS:000551550000019)】；

基金：P.-T.C. thanks the Ministry of Science and Technology (MOST) for financial support (107-2628-M-002-017). S.Y. and K.P. were supported by NATO Science for Peace and Security Programme-SPS 985291 and the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreements No. 690853 and No. 796245 (to S.Y.). Z.Z. thanks the financial support from the NSFC/China (21905090), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, and Natural Science Foundation of Shanghai (19ZR1412200).

语种：英文

外文关键词：Cell membranes - Cytology - Dynamics - Emission spectroscopy - Excited states - Fluorescence - Histology - Probes - Tissue

摘要：We demonstrate the construction of wavelength lambda-ratiometric images that allow visualizing the distribution of microscopic dynamics within living cells and tissues by using the newly developed principle of fluorescence response. The bent-to-planar motion in the excited state of incorporated fluorescence probes leads to elongation of the pi-delocalization, resultin in microviscosity-dependent but polarity-insensitive interplay between well-separated blue and red bands in emission spectra. This allows constructing the exceptionally contrasted images of cellular dynamics. Moreover, the application of probes with increased affinity toward biological membranes allowed detecting the differences in dynamics between the plasma membrane and intracellular membrane structures. Such lambda-ratiometric microviscosity imaging was extended for mapping the living tissues and observing their inflammation-dependent changes.