文献类型：期刊文献

英文题名：Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation

作者：Li, Yifeng[1];Qiu, Yongqiu[1];Zhang, Xu[1];Zhu, Minglong[1];Tan, Wensong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2019

卷号：6

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513081468);WOS:【SCI-EXPANDED(收录号:WOS:000466868400001)】；

基金：The authors are grateful for the financial support of this research by the Open Project Funding of the State Key Laboratory of Bioreactor Engineering of China.

语种：英文

外文关键词：Biohydrogen production; Condition optimization; Strain screening; Enterobacter aerogenes; Glycerol

摘要：Biohydrogen technology has drawn much attention due to its many advantages. However, it is still necessary to screen much more strains with stronger hydrogen-producing capacity for future commercialization processes. In this paper, a biohydrogen-producing strain Enterobacter aerogenes EB-06 was isolated, identified, and named. It could convert glycerol to biohydrogen by microorganism fermentation. The effects of oxygen content, initial pH, initial glycerol concentration, and initial nitrogen source content on biohydrogen production process were investigated. The results have shown that biohydrogen generation was more favorable under anaerobic conditions. The optimum specific biohydrogen production rate (Q(H2)) was obtained as 41.47mmol H-2/g DCWh at 40g/L initial glycerol concentration. The optimum volume H-2 yield (C-H2) was 83.76mmol H-2/L at initial pH 7.0. It was found that nitrogen source content (0-4g/L) could promote biohydrogen production and cell growth. The biohydrogen production of Enterobacter aerogenes EB-06 from glycerol was optimized by the orthogonal experimental design. The optimal yield coefficient of biohydrogen from glycerol fermentation (Y-H2/glycerol) of EB-06 was obtained as 1.07mmol H-2/ mol glycerol at 10g/L initial glycerol concentration, initial pH 5.0, and initial C/N ratio 5/3.