文献类型：期刊文献

英文题名：Transposon insertion sequencing analysis unveils novel genes involved in luxR expression and quorum sensing regulation in Vibrio alginolyticus

作者：Zhang, Yibei[1,3];Wu, Xiao[1];Cai, Jingxiao[1];Chen, Mo[1];Zhang, Jun[1];Shao, Shuai[1,3];Zhang, Yuanxing[2,3];Ma, Yue[1,3];Wang, Qiyao[1,3]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2023

卷号：267

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20225013247323);WOS:【SCI-EXPANDED(收录号:WOS:000909954900004)】；

基金：Acknowledgments This work was supported by grant from National Key Research and Development Program (2022YFE0101200), CARS for Marine Fish Culture Industry (CARS-47), and Fundamental Research Funds for the Central Universities (222201221733).

语种：英文

外文关键词：Vibrio alginolyticus; LuxR; Temperature; Transposon insertion sequencing (Tn-seq)

摘要：Vibrio alginolyticus is an important conditional pathogen of fish, shrimp, shellfish, and other marine aquaculture animals that causes huge economic losses to the marine aquaculture industries. Temperature has a significant influence on its quorum sensing (QS) system, which is essential for its various physiological functions. Using transposon insertion sequencing (Tn-seq) technology, we identified 218 putative regulatory factors of LuxR, the master regulator of QS in V. alginolyticus. In addition to established regulators, novel regulatory factors involved in LuxR expression are related to multiple processes. OmpH, 00189, TolC, VscY, and NirD are validated upstream regulatory factors of LuxR. Interestingly, OmpH and 00189 repress luxR expression at lower temperatures and activate its expression at higher temperatures. In contrast, TolC, VscY, and NirD enhance luxR expression at lower temperatures but suppress it at higher temperatures. Moreover, the abovementioned regulators are essential for QS-associated phenotypes, including Asp yields, motility, and biofilm formation, in temperaturedependent or temperature-independent manners. Thus, these novel regulators appear to relay various physiological signals in addition to temperature, effecting population phenotype modifications via QS regulation and warranting future investigation into the underlying mechanisms of opportunistic outbreaks of vibriosis.