文献类型：期刊文献

英文题名：Supramolecular Spherical Polyelectrolyte Brushes on Gold Nanoparticles for Light-Triggered Gene Delivery

作者：Liu, Liqun[1];Li, Yu[2,3];Zhu, Xinyu[2,3];Cui, Yuan[2,3];Zhou, Na[1];Guo, Xuhong[1,4];Li, Yueyue[1];Wang, Jie[1]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Chem Engn & Low Carbon Technol, Shanghai 200237, Peoples R China;[2]Soochow Univ, Affiliated Hosp 1, Dept Thorac Surg, Suzhou 215005, Jiangsu, Peoples R China;[3]Soochow Univ, Inst Minimally Invas Thorac Canc Therapy & Transla, Suzhou Med Coll, Suzhou 215000, Jiangsu, Peoples R China;[4]Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China

年份：2026

卷号：15

期号：1

起止页码：120

外文期刊名：ACS MACRO LETTERS

收录：;EI(收录号：20260419941989);WOS:【SCI-EXPANDED(收录号:WOS:001640407000001)】；

基金：We gratefully acknowledge the National Natural Science Foundation of China (Nos. 51403062 and 82500087) for support of this work. We gratefully thank financial support from the National Key R&D Program of China (2023YFD1700303), Suzhou Science and Education for Health Program (QNXM2024007), and the Science and Technology Innovation Program of Hunan Province (2024RC7001). The TOC figure was created in BioRender by Li, Y. (2025) (https://BioRender.com/z6quss9) and approved to publish (No. LU292TS3T9).

语种：英文

外文关键词：Cell death - Cell proliferation - Dynamic light scattering - Fiber optic sensors - Gene expression - Gene therapy - Gene transfer - Gold compounds - Metal nanoparticles - Molecular biology - Supramolecular chemistry

摘要：Spherical polyelectrolyte brushes (SPBs) have been of great interest in biomedicine, particularly in gene delivery, yet their brush architectures are commonly fixed, showing limited tunability and responsiveness. Herein, we report a light-controllable supramolecular SPB (AuNP@CDs@Azo-PD) constructed by assembling azobenzene-terminated poly(2-(dimethylamino)ethyl methacrylate) (Azo-PD) onto beta-cyclodextrin-functionalized gold nanoparticles (AuNP@CDs) via reversible host-guest interactions. The resulting nanostructure exhibits a core-shell architecture with a brush-like polymer corona. Characterization of the system through dynamic light scattering (DLS) and UV-vis spectroscopy confirmed the successful formation of the nanocarrier and its ability to undergo controlled structural transitions under UV irradiation. The SPB system was further evaluated for its gene delivery capability, showing excellent biocompatibility and effective plasmid DNA encapsulation, with protection against serum nucleases. In vitro transfection studies revealed that AuNP@CDs@Azo-PD achieved comparable transfection efficiency to Lipofectamine 3000, with efficient gene expression and suppression of the PI3K/AKT signaling pathway. Additionally, UV-triggered release of the PTEN gene resulted in enhanced tumor suppression effects, including reduced cell proliferation, migration, and increased apoptosis. These findings demonstrate the potential of AuNP@CDs@Azo-PD as a versatile and responsive nanoplatform for controlled gene delivery and cancer therapy.