文献类型：期刊文献

中文题名：罐组逆流提取荷叶生物碱的研究

英文题名：Extracting of alkaloids from Nelumbo nucifera leaves by multi-stage countercurrent extraction

作者：黄鑫[1];周永传[1];陈德煦[1]

机构：[1]华东理工大学中药现代化工程中心,上海200237

年份：2009

卷号：40

期号：8

起止页码：1229

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：荷叶;生物碱;罐组逆流提取;正交试验

外文关键词：the leaves of Nelumbo nucifera Gaertn. ; alkaloids; multi-stage countercurrent extraction; orthogonal test

摘要：目的采用罐组逆流提取技术提取荷叶中的荷叶生物碱并优化其工艺条件。方法采用分光光度法测定荷叶生物碱。按L16(44)正交设计表设计试验,分别考察提取时间、提取温度、固液比和乙醇体积分数4个因素的影响。结果通过极差分析得出罐组逆流提取荷叶生物碱的最佳工艺条件为:提取时间25 min,提取温度80℃,固液比1∶50,乙醇体积分数为70%。结论可以将罐组逆流提取技术应用于荷叶提取工程。
Objective To optimize the extracting process of alkaloid from Nelumbo nucifera leaves by multi-stage countercurrent extraction. Methods The spectrophotometry was used to determine the alkaloids. The extracting process of alkaloid was optimized by L16 （4^4 ） to observe the effect of extraction time, extraction temperature, solid to liquid ratio, and ethanol concentration, respectively. Results The optimum extraction parameters were extraction time 25 min, extraction temperation 90℃, solid to liquid ratio 1 ： 50, and ethanol concentration 70%. Conclusion The multi-stage countercurrent extraction is used to the extracting process of alkaloid from N. nucifera leaves.