文献类型：期刊文献

英文题名：A turn-on fluorescent probe for tumor hypoxia imaging in living cells

作者：Cai, Qi[1];Yu, Tao[1];Zhu, Weiping[1];Xu, Yufang[1];Qian, Xuhong[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2015

卷号：51

期号：79

起止页码：14739

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000361753000010)】；

基金：This work was supported by the financial support from National Natural Science Foundation of China (21476077 and 21236002), National Basic Research Program of China (973 Program, 2013CB733700), the National High Technology Research and Development Program of China (863 Program, 2011AA10A207), Shanghai Pujiang Program and the Fundamental Research Funds for the Central Universities.

语种：英文

摘要：A novel "nturn-on'' fluorescent probe HP for hypoxia imaging was designed and synthesized based on rhodamine B and a naphthalimide fluorophore. The fluorescence of HP is very weak owing to the FRET effect from rhodamine B to the azo-naphthalimide unit. Under hypoxia conditions, the azo-bond is reduced and the fluorescence at 581 nm enhances dramatically as a result of disintegration of the quencher structure. Verified by the cyclic voltammetry reduction potential and proposed product HPN, the probe HP could undergo the chemical and cytochrome P450 enzymatic reduction quickly. When cultured with HeLa cells, HP showed remarkable fluorescence differences at various oxygen concentrations, and the ratio of fluorescence intensity between hypoxic and normoxic cells could reach 9 fold.