文献类型：期刊文献

中文题名：海洋真菌灰绿曲霉蓝光感应基因Agwc1的克隆、鉴定与序列分析

英文题名：Cloning,Identification and Sequence Analysis of the Gene Agwc1 Required for Blue-light Sensing in Marine-derived Fungus Aspergillus glaucus

作者：方喆[1];周祥山[1];张健[1];张盈[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2010

卷号：26

期号：11

起止页码：191

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：灰绿曲霉;海洋丝状真菌;WC-1蛋白;蓝光感应;序列分析

外文关键词：Aspergillus glaucus Marine-drived filamentous fungus WC-1 protein Blue-light sensing Sequence analysis

摘要：蓝光感应系统在不同的光感应过程中都起到十分关键的作用,这些生理过程包括无性/有性发育,昼夜节律钟,次级代谢产物的生成等。为了对蓝光感应系统作进一步的研究,通过简并引物PCR和基因组走读方法得到了海洋真菌灰绿曲霉蓝光调节受体基因Agwc1,并通过多种生物信息学方法对其核苷酸和蛋白质序列进行了分析。Agwc1基因全长2724bp,包含3个内含子,编码852个氨基酸,蛋白质分子量是96kD,等电点为8.17,很有可能定位于细胞核中,通过序列比对与系统进化树分析,发现AgWC1蛋白与其他物种中的WC-1蛋白有很高的同源性。在本研究中成功鉴定了Agwc1基因,这是国际上首次在海洋丝状真菌领域鉴定光感应基因,并且AgWC1很有可能在灰绿曲霉蓝光感应系统中发挥重要功能。
Blue-light sensing play a key role in many different physiological responses,such as asexual conidiation,the circadian clock,secondary metabolism,pigmentation and sexual development.To further study blue-light sensing system,we isolated and characterized Agwc1,a gene encoding a putative blue-light regulator in marine-derived filamentous fungus Aspergillus glaucus（A.glaucus）by carrying out degenerate oligonucleotide PCR and gene walking,successively.Several kinds of bioinformatics methods were used to analyze information of protein and nucleotide about these four light regulators.The length of putative Agwc1 is 2 724 bp,containing three introns,encoding a 852 aa protein with MW 96 kD and pI 8.17.The AgWC1 protein possibly localized to the nucleus,sharing high similarity with other WC-1 protein which was confirmed by the phylogenetic tree proteins.In the present study,we successfully obtained and identified Agwc1 which is the first gene required for light-sensing in the field of marine-derived filamentous fungus.Furthermore,it is likely that AgWC1 play a key role in blue-light sensing system of A.glaucus.