文献类型：期刊文献

英文题名：An alpha/beta chimeric peptide molecular brush for eradicating MRSA biofilms and persister cells to mitigate antimicrobial resistance

作者：Xiao, Ximian[1];Zhang, Si[2];Chen, Sheng[2];Qian, Yuxin[2];Xie, Jiayang[2];Cong, Zihao[2];Zhang, Danfeng[2];Zou, Jingcheng[2];Zhang, Wenjing[2];Ji, Zhemin[2];Cui, Ruxin[2];Qiao, Zhongqian[2];Jiang, Weinan[2];Dai, Yidong[3];Wang, Yun[3];Shao, Xiaoyan[3];Sun, Yun[3];Xia, Jiang[4];Fei, Jian[5];Liu, Runhui[1,2]

机构：[1]East China Univ Sci & Thchnol, Sch Mat Sci & Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Mol Engn Polymers,Key Lab Ultrafine, Fudan Univ,Minist Educ,Res Ctr Biomed Mat,Frontie, Key Lab Specially Funct Polymer Mat & Related Tec, Shanghai, Peoples R China;[3]Shanghai Ruijin Rehabil Hosp, Shanghai 200023, Peoples R China;[4]Chinese Univ Hong Kong, Dept Chem, Hong Kong, Peoples R China;[5]Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Gen Surg, Shanghai 200025, Peoples R China

年份：2020

卷号：8

期号：24

起止页码：6883

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20205209679215);WOS:【SCI-EXPANDED(收录号:WOS:000600664500007)】；

基金：This research was supported by the National Natural Science Foundation of China (No. 21861162010, 21574038, 31800801), the Program of Shanghai Academic/Technology Research Leader (No. 20XD1421400), the National Key Research and Development Program of China (2016YFC1100401), the Research Program of State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities (No. 22221818014, 50321041917001, 222201814001), the Open Project of the State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University (No. SKLKF201909), Shanghai Collaborative Innovation Center for Translational Medicine (Grant No. TM201804), and the Medical Cross Fund Project of SJTU (No. YG2019ZDA17). The authors thank colleagues at the Research Center of Analysis and Test of East China University of Science and Technology for their help with the characterization. The authors also thank the staff members at the National Facility for Protein Science (NFPS) for providing technical support and assistance in data collection and analysis.

语种：英文

外文关键词：Dendrimers - Bacteria

摘要：Infections involving methicillin-resistant Staphylococcus aureus present great challenges, especially when biofilms and persister cells are involved. In this work, an alpha/beta chimeric polypeptide molecular brush (alpha/beta CPMB) is reported to show excellent performance in inhibiting the formation of biofilms and eradicating established biofilms. Additionally, the polymer brush efficiently killed metabolically inactive persister cells that are antibiotic-insensitive. Antimicrobial mechanism studies showed that alpha/beta CPMB causes membrane disturbance and a substantial increase in reactive oxygen species (ROS) levels to kill bacteria, and mesosome-like structure formation was also observed. Furthermore, the polymer brush was able to kill clinically isolated multidrug resistant Gram-positive bacteria with no risk of antimicrobial resistance. The alpha/beta CPMB has demonstrated great potential in addressing the great challenge of eradicating multidrug resistant Gram-positive bacterial infections.