文献类型：期刊文献

英文题名：Facile synthesis of glutathione-functionalized Fe3O4@polydopamine for separation of GST-tagged protein

作者：Ni, Kefeng[1];Yang, Jianbing[1];Ren, Yuhong[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：128

起止页码：392

外文期刊名：MATERIALS LETTERS

收录：;EI(收录号：20142217757945);WOS:【SCI-EXPANDED(收录号:WOS:000337876000101)】；

基金：This work was funded by the National Natural Foundation of China (No. 21076079), National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204) and the Fundamental Research Funds for the Central Universities and National Major Science.

语种：英文

外文关键词：Polydopamine; Glutathione; Magnetic materials; Adhesion; Protein separation

摘要：In this study, a glutathione-functionalized Fe3O4@polydopamine (GSH-PD-IONPs) was designed and synthesized via facile dopamine chemistry. The method involved in situ coating of Fe3O4 nanoparticles with polydopamine, followed by the conjugation of glutathione to the polydopamine film on the surface of the particle. GSH-PD-IONPs were used to separate the glutathione S-transferase (GST)-tagged green fluorescent protein (GFP) via the enzyme-substrate interactions between GST and GSH. GSH-PD-IONPs showed high selectivity toward GST-GFP fusion proteins and can be potentially used for protein purification. (C) 2014 Elsevier B.V. All rights reserved.