文献类型：期刊文献

英文题名：Preparative separation and purification of fumigaclavine C from fermented mycelia of Aspergillus fumigatus CY018 by macroporous adsorption resin

作者：Yao, Ling-Yun[1];Zhu, Yi-Xiang[1];Liu, Chang-Qing[1];Jiao, Rui-Hua[2];Lu, Yan-Hua[1];Tan, Ren-Xiang[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Nanjing Univ, Inst Funct Biomol, State Key Lab Pharmaceut Biotechnol, Nanjing 210008, Jiangsu, Peoples R China

年份：2015

卷号：989

起止页码：122

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20151300691144);WOS:【SCI-EXPANDED(收录号:WOS:000353081400016)】；

基金：The authors are grateful for funding from the National High Technology Research and Development Program of China (2011AA090702 and 2013AA092901), the Fundamental Research Funds for the Central Universities, the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204) as well as Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Adsorption macroporous resin; Aspergillus fumigatus; Fumigaclavine C; Purification; Thermodynamics

摘要：In this work, the separation and purification of fumigaclavine C (FC), an ergot alkaloid with strong anti-inflammatory activity from fermented mycelia of Aspergillus fumigatus was systematically evaluated. Among the eight tested resins, the non-polar resin D101 displayed the best adsorption and desorption based on of static adsorption and desorption tests. Adsorption isotherms were constructed on D101 resin and fitted well to the Freundlich model. Dynamic adsorption and desorption tests on a column packed with D101 resin have been investigated for optimization of chromatographic parameters. Under optimized conditions, the contents of FC increased from 7.32% (w/w) in the crude extract to 67.54% in the final product with a recovery yield of 90.35% (w/w) via one run. Furthermore, a lab scale-up separation was carried out, in which the FC content and recovery yield were 65.83% and 90.13%, respectively. These results demonstrated that this adsorption-desorption strategy by using D101 resin was simple and efficient, thus showing potential for large scale purification and preparation of FC in the future. (C) 2014 Elsevier B.V. All rights reserved.