文献类型：期刊文献

中文题名：基于Euler-Lagrange框架的生物反应过程的数值模拟研究

英文题名：Numerical simulation of bioreaction process based on Euler-Lagrange frame

作者：林骥晗[1];夏建业[1];储炬[1];庄英萍[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2016

卷号：44

期号：6

起止页码：4

中文期刊名：化学工程

外文期刊名：Chemical Engineering(China)

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金青年基金(21506052);中荷国际合作项目(2013DFG32630);863计划项目(2014AA021701);中央高校基本科研业务探索基金(222201414037)

语种：中文

中文关键词：OpenFOAM;欧拉-拉格朗日;搅拌釜式反应器;模型结合;放大效应

外文关键词：OpenFOAM; Euler-Lagrange; stirred-tank bioreactor; model integration; scale-up effect

摘要：工业规模反应器内部出现的底物浓度、溶氧等环境条件非均一性分布会对菌群的生理代谢产生不利影响,进而引起产品产率下降、副产物生成。研究反应器内非均一性影响的方法主要可分为实验法和模拟法。文中主要建立了一种基于OpenFOAM的计算流体力学和生物反应动力学模型耦合的计算模拟方法,为生物过程高效放大提供模拟工具。构建了基于Euler-Lagrange方法的耦合模拟方法,实现了速度场控制的物质输运、速度场控制的粒子运动、粒子读取环境浓度信息、耦合求解描述细胞动力学模型的微分方程组、粒子浓度信息反馈给环境浓度场等功能。最后将描述地衣芽孢杆菌发酵代谢产酸的动力学模型整合到搭建的框架中,通过5 L反应器内的批发酵模拟验证了新方法在计算流程上的可行性。在此基础上进行了批发酵和补料分批发酵反应器内部环境非均一性的初步探索,通过50 L反应器内补料发酵的模拟,初步研究了长时间的底物浓度非均一性对菌群代谢的累积影响。
The heterogeneity of spatial gradients such as substrate gradients or oxygen gradients in large-scale bioreactors usually has an adverse impact on cell metabolism,leading to low yields and increasing formation of by-products. There are two categories of methodologies to study the bioreactor heterogeneity： experimental methodology and simulation methodology. A model integration method based on OpenFOAM was established,which provided a simulation tool for rapid development of bioprocesses. The model integration method was based on EulerLagrange approach. Compared to experimental methodology,this method can provide more details about the flow field. Basic functions,such as substrate transport,particle movement,coupling between extra-concentrations and intra-concentrations can also be provided. The dynamic model of acid production by Bacilluslic beniformisis integrated into the formed integration frame to verify its feasibility. The combined model showed similar simulation result as the original model when it was applied to batch fermentation in a 5 L reactor. Furthermore,the environmental heterogeneity of the batch reactor and fed-batch fermentation were explored preliminarily. The preliminary study on long-time effect of environmental heterogeneity on cell population was conducted via a fedbatch simulation in a 50 L bioreactor.