文献类型：期刊文献

英文题名：A hydrolytically stable amide polar stationary phase for hydrophilic interaction chromatography

作者：Li, Zongying[1];Li, Shuxiang[1];Zhang, Feifang[1];Geng, Huiliang[1];Yang, Bingcheng[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai 200237, Peoples R China

年份：2021

卷号：231

外文期刊名：TALANTA

收录：;EI(收录号：20211510192478);WOS:【SCI-EXPANDED(收录号:WOS:000658306800010)】；

基金：This work was sponsored by National Key Research and Development Program of China (2018YFC1603500) and National Natural Science Foundation of China (No. 21974044).

语种：英文

外文关键词：Hydrophilic interaction chromatography; Stationary phase; Amide; Polymer; Glycidyl methacrylate; Bleed

摘要：A novel amide functionalized polar stationary phase has been described by grafting acrylamide polymer coating onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microsphere. The grafting of acrylamide coating is performed via free radical polymerization of acrylamide with pendant double bonds of hydrolysed GMA-DVB microsphere. The obtained stationary phase (G-pAM) possesses favorable hydrophilicity, as proved by strong retention and good separation ability towards several types of model polar analytes (e.g. 71913 plates/m plate count of sucrose), and excellent hydrolytically stability, as indicated by extremely low bleed level (much superior to commercial ones, similar to 23.7-fold-similar to 77.4-fold lower). Negligible baseline drift under gradient elution (water fraction even up to 50%) was observed. It also exhibits good selectivity in the separation of isomers and homologue sugars.