文献类型：期刊文献

英文题名：A well defect-suitable and high-strength biomimetic squid type II gelatin hydrogel promoted in situ costal cartilage regeneration via dynamic immunomodulation and direct induction manners

作者：Dai, Meilu[1];Sui, Baiyan[1];Hua, Yujie[2];Zhang, Yiqing[2];Bao, Bingkun[2];Lin, Qiuning[2];Liu, Xin[1];Zhu, Linyong[2];Sun, Jiao[1]

机构：[1]Shanghai Jiao Tong Univ, Peoples Hosp 9, Shanghai Biomat Res & Testing Ctr, Shanghai Key Lab Stomatol,Sch Med, Shanghai 200023, Peoples R China;[2]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：240

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20200808208147);WOS:【SCI-EXPANDED(收录号:WOS:000523562900003)】；

基金：This work was sponsored by grants from the National Key Research and Development Program of China (2018YFC1105202), the Shanghai Pujiang Program (18PJD024), the National Nature Science Foundation of China (21774030), the Science and Technology Commission of Shanghai Municipality (18DZ2290300) and the Shanghai Sailing Program (19YF1425700).

语种：英文

外文关键词：Squid type II gelatin; Double network hydrogel; Dynamic immunomodulation; Neutrophil; Macrophage; Costal cartilage regeneration

摘要：Reconstructing segmental costal cartilage defects resulting from autologous cartilage grafts in plastic surgery remains a challenge. The present study focused on a biomimetic strategy for in situ costal cartilage regeneration that did not rely on an autogenous/xenogenous tissue graft. A multifunctional biomimetic SGII/HA-DN hydrogel based on a "chemical-curing, shaping, and light-curing" gelation system was developed and evaluated for its mechanical properties, clinical applications and biological functions. This hydrogel showed good suitability to repair defects and a high mechanical support strength (11 MPa, which is close to the natural strength of costal cartilage). Biologically, the hydrogel exhibited dual-immunomodulatory effects on the pro-inflammatory/antiinflammatory phenotypes of neutrophils and M1/M2 macrophage polarization and subsequently promoted the chondrogenesis of cartilage stem/progenitor cells through both direct induction and indirect stimulation by the M2 macrophage-mediated TGF-beta/Smad pathway. Furthermore, this SGII/HA-DN hydrogel could regulate the local microenvironment, inducing new costal cartilage regeneration in vivo. Our findings demonstrate that the newly developed multifunctional SGII/HA-DN hydrogel provides a strategy with high prospect for the biomimetic repair of segmental costal cartilage defects in clinical practice.