文献类型：期刊文献

英文题名：An efficient approach based on basic strong cation exchange chromatography for enriching methylated peptides with high specificity for methylproteomics analysis

作者：Li, Zhouxian[1,2];Wang, Qi[2,3];Wang, Yan[2,3];Wang, Keyun[2,3];Liu, Zhen[2,3];Zhang, Weibing[1];Ye, Mingliang[2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Dept Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2021

卷号：1161

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20211510196132);WOS:【SCI-EXPANDED(收录号:WOS:000637930800001)】；

基金：This work was supported, in part, by funds from Science and Technology Commission of Shanghai Municipality (19142201100), the National Key Research and Development Program of China (2020YFE0202200, 2016YFA0501402, 2017YFA0505004), the National Natural Science Foundation of China (21974045, 21675061, 91753105), the Fundamental Research Funds for the Central Universities (50321102117022), the LiaoNing Revitalization Talents Program, the innovation program (DICP I201935, DICP&QIBEBT UN201802) of science and research from the DICP, CAS, and Innovation Academy for Precision Measurement Science and Technology, CAS.

语种：英文

外文关键词：Protein methylation; Multiple enzymes digestion; Carboxylic amidation; Basic strong cation exchange chromatography

摘要：Protein methylation as one of the most important post-translational modifications has been under the spotlight due to its essential role in many biological processes. Development of methods for large-scale analysis of protein methylation greatly accelerates the related researches. To date, antibody-based enrichment strategy is the most common approach for methylproteomics analysis. However, it is still lacking of a pan-specific antibody to enrich peptides or proteins carrying all kinds of lysine and arginine methylation forms. Herein, an online basic strong cation exchange chromatography was developed to enrich methylated peptides from protein digests prepared by two complementary methods, including direct multiple enzymes digestion and carboxylic amidation followed by multiple enzymes digestion. After enrichment, the majority of identifications were obtained from direct multiple enzymes digested sample. The enrichment specificity of methylated peptides was up to 28.5%, and 445 methylation forms corresponding to 376 methylation sites were identified on 194 proteins in one LC-MS/MS run using only 100 mu g of digests. This method has great potential in studying protein methylation mediated biological processes. (C) 2021 Elsevier B.V. All rights reserved.