文献类型：期刊文献

中文题名：应用高速逆流一次性分离川芎中的蛇床内酯A和Z-蒿本内酯

英文题名：One step separation and preparation of senkyunolide A and Z-ligustilide in Ligusticum chuanxiong Hort by high speed counter current chromatography

作者：刘雯[1];吴平丽[1];卓超[1];张继全[2];沈平孃[2]

机构：[1]华东理工大学药学院,上海200237;[2]国家中药制药工程技术研究中心,上海201203

年份：2010

卷号：32

期号：5

起止页码：764

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家科技部国际科技合作项目--戒毒中药"济泰片"方剂的配伍规律及防复吸的机理研究(2006DFA32720)

语种：中文

中文关键词：高速逆流色谱;川芎;蛇床内酯A;蒿本内酯

外文关键词：high speed counter current chromatography; Ligusticum chuanxiong Hort; senkyunolide A; Z-ligustilide

摘要：目的:研究高速逆流色谱分离川芎活性成分的方法。方法:应用高速逆流技术一次性从川芎挥发油中分离出主要成分蛇床内酯A和Z-蒿本内酯。采用两相溶剂系统正己烷-乙酸乙酯-乙醇-水的四元体系,上相为固定相,下相为流动相,转速为892RPM,流速为1.2mL/min。结果:经过EI-MS和1H-NMR的鉴定,可以分别确认是蛇床内酯A和Z-蒿本内酯,纯度可以达到95%。结论:高速逆流色谱技术适合应用于分离内酯类这种极性较小的成分,分离度好,产物纯度较高。
AIM： To research how to separate the active component in Ligusticum chuanxiong Hort by high speed counter current chromatography. METHODS： Senkyunolide A and Z-ligustilide,the main components of volatile oil in Ligusticum chuanxiong Hort were one step separated by high speed counter current chromatography. n-hexane-ethyl acetate-ethanol-water,1 ∶ 1 ∶ 1 ∶ 1（v/v）,was used as the solvent system for HSCCC. Top phase and bottom phase were respectively used as static phase and mobile phase. Optimum speed and flow rate were 900 r/min and 1. 2 mL/min respectively. RESULTS： Collected fractions were analyzed by HPLC and identified by EI-MS and 1HNMR. Purity could reach more than 95% . CONCLUSION： Lactone is fit to be separated and prepared by high speed counter current chromatography with good resolution and high purity. We find a fast and efficient way to separate these.