文献类型：期刊文献

英文题名：Phytosphingosine promotes intracellular microbial lipid accumulation and cell structure remodeling during adaptive evolution combined with cell fractionation in wild-type Yarrowia lipolytica DSM 3286

作者：Li, Shaokai[1];Pan, Lixia[2];Zhang, Bin[1];Bao, Jie[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Guangxi Acad Sci, Natl Key Lab Nonfood Biomass Energy Technol, Nanning 530007, Peoples R China

年份：2026

卷号：234

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20262520966613);WOS:【SCI-EXPANDED(收录号:WOS:001801551400001)】；

基金：This research was supported by National Key R & D Program of China (2023YFA0914400) , National Natural Science Foundation of China (32301269) , Guangxi Science and Technology Challenge Project (Guike JB2504850001) , and the Key Technology R & D Program of the Science and Technology Commission of Shanghai Municipality (25HC2820200) .

语种：英文

外文关键词：Microbial lipid; Phytosphingosine; Adaptive evolution; Yarrowia lipolytica; Centrifugal fractionation

摘要：Adaptive laboratory evolution (ALE) based on cell density fractionation has emerged as a promising strategy to enhance intracellular lipid accumulation in oleaginous microorganisms, but it suffers from low efficiency. An ALE combined with centrifugal fractionation (ALECF) developed in our previous studies not only increased the intracellular lipid content in typical oleaginous yeasts, but also changed their cell structures after more than 20 serial transfers. In this study, phytosphingosine, a bioactive long-chain amino alcohol involved in yeast cell wall integrity signaling pathway, was introduced to accelerate ALECF in wild-type oleaginous yeast Yarrowia lipolytica DSM 3286.The results showed that the lipid content in Y. lipolytica increased from 31.2% to 64.8% (w/w) after only 2 transfers, and further reached 77.2% after 8 transfers. The phytosphingosine-treated evolved cells exhibited a yeast-like morphology, with the average cell volume increasing 6.9-fold compared to the cells without phytosphingosine treatment. Meanwhile, the main cell wall components, including glucan, mannan, and chitin, decreased by 49.2%, 33.1%, and 37.2%, respectively. Transcriptional analysis revealed that phytosphingosine changed the expression levels of the genes involved in sphingolipid metabolism, cell wall integrity, and carbon metabolism in Y. lipolytica. This study provides a novel phytosphingosine-treated ALECF method to enhance the lipid content in wild-type Y. lipolytica and offers potential targets for future targeted metabolic engineering.