文献类型：期刊文献

英文题名：High-Preservation Single-Cell Operation through a Photo-responsive Hydrogel-Nanopipette System

作者：Li, Zi-Yuan[1,2];Liu, Ying-Ya[1,2];Li, Yuan-Jie[1,2];Wang, Wenhui[1,2];Song, Yanyan[1,2];Zhang, Junji[1,2];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：60

期号：10

起止页码：5157

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20210309784020);WOS:【SCI-EXPANDED(收录号:WOS:000608159200001)】；

基金：The authors acknowledge the financial support from the NSFC (21878086, 21788102, 22020102006), the Shanghai Rising-Star Program (19QA1402500 to J.Z.). The project was supported by Shanghai Municipal Science and Technology Major Project (Grant No.2018SHZDZX03), the international cooperation program of Shanghai Science and Technology Committee (17520750100) and the Fundamental Research Funds for the Central Universities (222201717003). Z.L. acknowledges the financial support from Shanghai Sailing Program (20YF1410300); China Postdoctoral Science Foundation (2020M671017).

语种：英文

外文关键词：diarylethene; potential-free nanopipette; precise dosing; single-cell operation; supramolecular hydrogel

摘要：Single-cell and in situ cell-based operation with nanopipette approach offers a possibility to elucidate the intracellular processes and may aid the improvement of therapy efficiency and precision. We present here a photo-responsive hydrogel-nanopipette hybrid system that can achieve single-cell operation with high spatial/temporal resolution and negligible cell damage. This strategy overcomes long-time obstacles in nanopipette single-cell studies as high electric potential (ca. 1000 mV) or organic solvent is always used during operations, which would inevitably impose disturbance and damage to targeted cells. The light-triggered system promotes a potential-free, non-invasive single-cell injection, resulting in a well-retained cell viability (90 % survival rate). Moreover, the photo-driven injection enables a precisely dose-controllable single-cell drug delivery. Significantly reduced lethal doses of doxorubicin (163-217 fg cell(-1)) are demonstrated in corresponding cell lines.