文献类型：期刊文献

英文题名：Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources

作者：Xie, JL; Zhang, L; Ye, Q; Zhou, QW; Xin, L; Du, P; Gan, RB

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

年份：2003

卷号：25

期号：2

起止页码：173

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000180344400015)】；

语种：英文

外文关键词：angiostatin; dissolved O-2; glycerol feeding; Pichia pastoris

摘要：A recombinant strain of Pichia pastoris with a phenotype of Mut(S) was used to produce angiostatin. Due to the low methanol consumption rate of this strain, both methanol and glycerol feedings, that produced oscillation in dissolved O-2 concentration, were used during the expression phase to improve cell growth and angiostatin expression. However, enhanced cell growth led to nitrogen limitation that suppressed further production of angiostatin, but addition of ammonia allowed angiostatin concentration to reach 108 mg l(-1) after an expression period of 96 h. The ratio of consumed glycerol to methanol of 1.5:1 (w/w) in the expression phase suggested that methanol played an important role in the metabolism of carbon sources.