文献类型：期刊文献

英文题名：Preparation of (R)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp ECU0401

作者：He, Yu-Cai[1];Xu, Jian-He[1];Pan, Jiang[1];Ouyang, Li-Ming[1];Xu, Yi[1]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Bioproc, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：31

期号：5

起止页码：445

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000257594100008)】；

语种：英文

外文关键词：(R)-(-)-mandelic acid; Alcaligenes sp; ECU0401; (S)-(+)-mandelic acid dehydrogenase; enantioselective degradation; optical resolution

摘要：An enantioselective mandelate-degrading bacterium, Alcaligenes sp. ECU0401, was newly isolated from soil. By fed-batch culture, (R)-(-)-mandelic acid was successfully prepared in a 5-L fermenter with 32.8% isolated yield and > 99.9% enantiomeric excesses (e.e.) from totally 3.04% (w/v) of racemic mandelic acid after 99 h of biotransformation. The optimal reaction pH and temperature were 6.5 and 30 degrees C, respectively. Using the resting cell as a biocatalyst for asymmetric degradation of racemic mandelic acid and chloro-substituted derivatives thereof, (R)-(-)-mandelic acid, (R)-(-)-o-chloromandelic acid, (S)-(+)-m-chloromandelic acid and (S)-(+)-p-chloromandelic acid were recovered with high analytic yields and excellent enantiomeric excesses (e.e. > 99.9%). (R)-(-)-Mandelic acid could also be obtained after 12 h of biotransformation with 41.5% isolated yield and > 99.9% e.e.